CD223 antibody | C9B7W

Rat anti Mouse CD223

Product Type
Monoclonal Antibody
Clone
C9B7W
Isotype
IgG1
Specificity
CD223

Product Code Applications Pack Size List Price Your Price Qty
MCA2386
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
MCA2386GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223.

Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.

Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Murine CD223 Ig fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Source Reference

  1. Workman, C.J. et al. (2002) Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3).
    Eur J Immunol. 32 (8): 2255-63.

References for CD223 antibody

  1. Workman, C.J. et al. (2002) Cutting edge: molecular analysis of the negative regulatory function of lymphocyte activation gene-3.
    J Immunol. 169 (10): 5392-5.
  2. Workman, C.J. & Vignali, D.A. (2005) Negative regulation of T cell homeostasis by lymphocyte activation gene-3 (CD223).
    J Immunol. 174 (2): 688-95.
  3. Byrne, K.T. et al. (2011) Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma.
    J Clin Invest. 121 (5): 1797-809.
  4. Ordway, D. et al. (2007) The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation.
    J Immunol. 179: 522-31.
  5. Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
    J Immunol. 191 (1): 312-22.
  6. Iwasaki, Y. et al. (2013) Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.
    Eur J Immunol. 43: 1063-73.
  7. Woo, S.R. et al. (2010) Differential subcellular localization of the regulatory T-cell protein LAG-3 and the coreceptor CD4.
    Eur J Immunol. 40: 1768-77.

Flow Cytometry

Synonyms
LAG-3
RRID
AB_566651
UniProt
Q61790
Entrez Gene
Lag3
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042289 MHC class II protein binding
GO:0045085 negative regulation of interleukin-2 biosynthetic process
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
GO:0050868 negative regulation of T cell activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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