CD223 antibody | C9B7W
Rat anti Mouse CD223:Alexa Fluor® 488
Rat anti Mouse CD223:Alexa Fluor® 647
Rat anti Mouse CD223:Low Endotoxin
Rat anti Mouse CD223
Rat anti Mouse CD223:RPE
- Product Type
- Monoclonal Antibody
- Clone
- C9B7W
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2386A488T | F | 25 Tests/0.25ml |
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| MCA2386A488 | F | 100 Tests/1ml |
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| MCA2386A647 | F | 100 Tests/1ml |
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| MCA2386A647T | F | 25 Tests/0.25ml |
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| MCA2386EL | F FN | 0.5 mg |
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| MCA2386 | F | 0.25 mg |
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| MCA2386GA | F | 0.1 mg |
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| MCA2386PE | F | 100 Tests |
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Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223.
Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.
Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).
Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.
Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Murine CD223 Ig fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q61790
- Entrez Gene
- Lag3
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042289 MHC class II protein binding
- GO:0045085 negative regulation of interleukin-2 biosynthetic process
- GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
- GO:0050868 negative regulation of T cell activation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD223 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/200 | |
| Functional Assays | |||
| Flow Cytometry | 1/50 | 1/200 | |
| Flow Cytometry | Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
Source Reference
-
Workman, C.J. et al. (2002) Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3).
Eur J Immunol. 32 (8): 2255-63.
References for CD223 antibody
-
Workman, C.J. et al. (2002) Cutting edge: molecular analysis of the negative regulatory function of lymphocyte activation gene-3.
J Immunol. 169 (10): 5392-5. -
Workman, C.J. & Vignali, D.A. (2005) Negative regulation of T cell homeostasis by lymphocyte activation gene-3 (CD223).
J Immunol. 174 (2): 688-95. -
Byrne, K.T. et al. (2011) Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma.
J Clin Invest. 121 (5): 1797-809. -
Ordway, D. et al. (2007) The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation.
J Immunol. 179: 522-31. -
Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
J Immunol. 191 (1): 312-22. -
Iwasaki, Y. et al. (2013) Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.
Eur J Immunol. 43: 1063-73. -
Woo, S.R. et al. (2010) Differential subcellular localization of the regulatory T-cell protein LAG-3 and the coreceptor CD4.
Eur J Immunol. 40: 1768-77.
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