CD223 antibody | C9B7W
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F ResetRat anti Mouse CD223
- Product Type
- Monoclonal Antibody
- Clone
- C9B7W
- Isotype
- IgG1
- Specificity
- CD223
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Murine CD223 Ig fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Source Reference
-
Workman, C.J. et al. (2002) Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3).
Eur J Immunol. 32 (8): 2255-63.
References for CD223 antibody
-
Workman, C.J. et al. (2002) Cutting edge: molecular analysis of the negative regulatory function of lymphocyte activation gene-3.
J Immunol. 169 (10): 5392-5. -
Workman, C.J. & Vignali, D.A. (2005) Negative regulation of T cell homeostasis by lymphocyte activation gene-3 (CD223).
J Immunol. 174 (2): 688-95. -
Byrne, K.T. et al. (2011) Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma.
J Clin Invest. 121 (5): 1797-809. -
Ordway, D. et al. (2007) The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation.
J Immunol. 179: 522-31. -
Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
J Immunol. 191 (1): 312-22. -
Iwasaki, Y. et al. (2013) Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.
Eur J Immunol. 43: 1063-73. -
Woo, S.R. et al. (2010) Differential subcellular localization of the regulatory T-cell protein LAG-3 and the coreceptor CD4.
Eur J Immunol. 40: 1768-77.
- Synonyms
- LAG-3
- RRID
- AB_566651
- UniProt
- Q61790
- Entrez Gene
- Lag3
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042289 MHC class II protein binding
- GO:0045085 negative regulation of interleukin-2 biosynthetic process
- GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
- GO:0050868 negative regulation of T cell activation
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