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CD22 antibody | OX-97

Rat anti Mouse CD22

Product Type
Monoclonal Antibody
Clone
OX-97
Isotype
IgG1
Specificity
CD22

Product Code Applications Pack Size List Price Your Price Qty
MCA2187
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader

Rat anti Mouse CD22 antibody, clone OX-97 recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/poistive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD22 antibody

  1. Bakker, T.R. et al. (2002) Comparison of CD22 binding to native CD45 and synthetic oligosaccharide.
    Eur J Immunol. 32 (7): 1924-32.
  2. Duong, B.H. et al. (2010) Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.
    J Immunol. 184: 4143-58.

Synonyms
Lyb-8
RRID
AB_324218
UniProt
P35329
Entrez Gene
Cd22
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0015026 coreceptor activity

MCA2187

150128 159361

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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