CD200R antibody | OX-110
Rat anti Mouse CD200R:Alexa Fluor® 488
Rat anti Mouse CD200R:Alexa Fluor® 647
Rat anti Mouse CD200R:FITC
Rat anti Mouse CD200R:Low Endotoxin
Rat anti Mouse CD200R
Rat anti Mouse CD200R:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-110
- Isotype
- IgG2a
Rat anti Mouse CD200R antibody, clone OX-110 recognizes mouse CD200R, a cell surface glycoprotein (also known as OX2R. Mouse CD200R is a 326 amino acid, ~48 kDa single pass type I transmembrane glycoprotein, expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells (Wright et al.2003). Studies suggest that CD200-CD200R interaction may be involved in the control of myeloid cellular function (Minas and Liversidge 2006).
Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008).
Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None Present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Fusion protein mCD200RCD4d3+4
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsStore at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q9ES57 Related reagents
- Entrez Gene
- Cd200r1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD200R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/2 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/10 Pack Size: 25 µg, 0.1 mg 1/20 Pack Size: 0.5 mg |
|
| Flow Cytometry | 1/25 | 1/50 | |
| Flow Cytometry | 1/25 | 1/50 | |
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 488 | MCA1212A488 | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control:FITC | MCA1212F | 100 Tests | F | ||
| Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | 0.5 mg | F | ||
| Rat IgG2a Negative Control | MCA1212 | 1 ml | E F | ||
| Rat IgG2a Negative Control:RPE | MCA1212PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
References for CD200R antibody
-
Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
J Immunol. 171 (6): 3034-46. -
Zhao, J. et al. (2009) Evasion by stealth: inefficient immune activation underlies poor T cell response and severe disease in SARS-CoV-infected mice.
PLoS Pathog. 5 (10): e1000636. -
Björck, P. et al. (2011) Plasmacytoid Dendritic Cell Dichotomy: Identification of IFN-{alpha} Producing Cells as a Phenotypically and Functionally Distinct Subset.
J Immunol. 186: 1477-85. -
Dellacasa-Lindberg, I. et al. (2011) Migratory Activation of Primary Cortical Microglia upon Infection with Toxoplasma gondii.
Infect Immun. 79: 3046-52. -
Krejsek, J. et al. (2010) Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation.
Perfusion 25: 389-97. -
Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
Mol Immunol. 45: 1126-35. -
Costello, D.A. et al. (2011) Long term potentiation is impaired in membrane glycoprotein CD200-deficient mice: a role for Toll-like receptor activation.
J Biol Chem. 286 (40): 34722-32. -
Simelyte, E. et al. (2008) CD200-Fc, a novel antiarthritic biologic agent that targets proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis.
Arthritis Rheum. 58: 1038-43. -
Whitmore, L.C. et al. (2014) NOX2 protects against progressive lung injury and multiple organ dysfunction syndrome.
Am J Physiol Lung Cell Mol Physiol. 307: L71-82. -
Chitnis, T. et al. (2007) Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration.
Am J Pathol. 170: 1695-712. -
Larson, D. et al. (2012) Chronic helminth infection reduces basophil responsiveness in an IL-10-dependent manner.
J Immunol. 188: 4188-99. -
Torrero, M.N. et al. (2009) CD200R surface expression as a marker of murine basophil activation.
Clin Exp Allergy. 39 (3): 361-9. -
Hernangómez M et al. (2012) CD200-CD200R1 interaction contributes to neuroprotective effects of anandamide on experimentally induced inflammation.
Glia 60 (9): 1437-50. -
Bain, C.C. & Mowat, A.M. (2012) CD200 receptor and macrophage function in the intestine.
Immunobiology 217 (6): 643-51. -
Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
Int Immunopharmacol. 11 (7): 794-801. -
Akkaya, M. et al. (2013) Dissection of agonistic and blocking effects of CD200 receptor antibodies.
PLoS One 8 (5): e63325. -
Cassard L et al. (2012) Fcγ receptors inhibit mouse and human basophil activation.
J Immunol. 189 (6): 2995-3006. -
Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
J Immunol. Jul 6. pii: 1600052. [Epub ahead of print] -
Toya, E.E. & Ohba, M. (2015) Improved Long-term Culture of Epidermal Stem Cells Utilizing CD200R-expressing Feeder Cells
Showa Uni J Med Sci 27 (2) 83-91
