CD200R antibody | OX-110

Rat anti Mouse CD200R

Product Type
Monoclonal Antibody
Clone
OX-110
Isotype
IgG2a
Specificity
CD200R

Product Code Applications Pack Size List Price Your Price Qty
MCA2281GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
MCA2281
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD200R antibody, clone OX-110 recognizes mouse CD200R, a cell surface glycoprotein (also known as OX2R. Mouse CD200R is a 326 amino acid, ~48 kDa single pass type I transmembrane glycoprotein, expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells (Wright et al.2003). CD200-CD200R interaction may be involved in the control of myeloid cellular function (Minas and Liversidge 2006).

Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Fusion protein mCD200RCD4d3+4
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Rat IgG2a Negative Control

References for CD200R antibody

  1. Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
    J Immunol. 171 (6): 3034-46.
  2. Zhao, J. et al. (2009) Evasion by stealth: inefficient immune activation underlies poor T cell response and severe disease in SARS-CoV-infected mice.
    PLoS Pathog. 5 (10): e1000636.
  3. Björck, P. et al. (2011) Plasmacytoid Dendritic Cell Dichotomy: Identification of IFN-{alpha} Producing Cells as a Phenotypically and Functionally Distinct Subset.
    J Immunol. 186: 1477-85.
  4. Dellacasa-Lindberg, I. et al. (2011) Migratory Activation of Primary Cortical Microglia upon Infection with Toxoplasma gondii.
    Infect Immun. 79: 3046-52.
  5. Krejsek, J. et al. (2010) Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation.
    Perfusion 25: 389-97.
  6. Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
    Mol Immunol. 45: 1126-35.
  7. Costello, D.A. et al. (2011) Long term potentiation is impaired in membrane glycoprotein CD200-deficient mice: a role for Toll-like receptor activation.
    J Biol Chem. 286 (40): 34722-32.
  8. Simelyte, E. et al. (2008) CD200-Fc, a novel antiarthritic biologic agent that targets proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis.
    Arthritis Rheum. 58: 1038-43.
  9. View The Latest Product References
  10. Whitmore, L.C. et al. (2014) NOX2 protects against progressive lung injury and multiple organ dysfunction syndrome.
    Am J Physiol Lung Cell Mol Physiol. 307: L71-82.
  11. Chitnis, T. et al. (2007) Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration.
    Am J Pathol. 170: 1695-712.
  12. Larson, D. et al. (2012) Chronic helminth infection reduces basophil responsiveness in an IL-10-dependent manner.
    J Immunol. 188: 4188-99.
  13. Torrero, M.N. et al. (2009) CD200R surface expression as a marker of murine basophil activation.
    Clin Exp Allergy. 39 (3): 361-9.
  14. Hernangómez M et al. (2012) CD200-CD200R1 interaction contributes to neuroprotective effects of anandamide on experimentally induced inflammation.
    Glia 60 (9): 1437-50.
  15. Bain, C.C. & Mowat, A.M. (2012) CD200 receptor and macrophage function in the intestine.
    Immunobiology 217 (6): 643-51.
  16. Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
    Int Immunopharmacol. 11 (7): 794-801.
  17. Akkaya, M. et al. (2013) Dissection of agonistic and blocking effects of CD200 receptor antibodies.
    PLoS One 8 (5): e63325.
  18. Cassard L et al. (2012) Fcγ receptors inhibit mouse and human basophil activation.
    J Immunol. 189 (6): 2995-3006.
  19. Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
    J Immunol. Jul 6. pii: 1600052. [Epub ahead of print]
  20. Toya, E.E. & Ohba, M. (2015) Improved Long-term Culture of Epidermal Stem Cells Utilizing CD200R-expressing Feeder Cells
    Showa Uni J Med Sci 27 (2) 83-91.

Flow Cytometry

Immunohistology - Frozen

Western Blotting

Synonyms
OX2R
RRID
AB_566622
UniProt
Q9ES57
Entrez Gene
Cd200r1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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