CD1d antibody | 1B1

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Rat anti Mouse CD1d:FITC

Rat anti Mouse CD1d

Rat anti Mouse CD1d:RPE

Product Type
Monoclonal Antibody
Clone
1B1
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4700F F 0.1 mg
MCA4700 C F IP 0.25 mg
MCA4700PE F 0.1 mg
Rat anti Mouse CD1d antibody, clone 1B1 recognizes mouse CD1d, a glycoprotein expressed on cortical thymocytes, certain T cell leukemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse CD1d cDNA-transfected RMA-S mouse T lymphoma
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch

More Information

UniProt
P11609 Related reagents
Entrez Gene
Cd1d1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0005769 early endosome
GO:0005770 late endosome
GO:0009897 external side of plasma membrane
GO:0010008 endosome membrane
GO:0030883 endogenous lipid antigen binding
GO:0045059 positive thymic T cell selection
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045087 innate immune response
GO:0045404 positive regulation of interleukin-4 biosynthetic process
GO:0048007 antigen processing and presentation, exogenous lipid antigen via MHC class Ib
GO:0051135 positive regulation of NK T cell activation
GO:0051138 positive regulation of NK T cell differentiation
Regulatory
For research purposes only

Applications of CD1d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for CD1d antibody

  1. Sun, J. et al. (2010) Deficiency of antigen-presenting cell invariant chain reduces atherosclerosis in mice.
    Circulation 122 (8): 808-20.
  2. Zhou, Z. et al. (2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
    BMC Immunol. 12: 7.
  3. Soh, S. Y. et al. (2016) NKT Cell Hyporesponsiveness Leads to Unrestrained Accumulation of Marginal Zone B Cells in Hypercholesterolemic Apolipoprotein E-Deficient Mice.
    The Journal of Immunology. Oct 21 [Epub ahead of print].