CD1d antibody | 1B1
Rat anti Mouse CD1d antibody, clone 1B1 (MCA4700) used for immunofluorescence staining of mouse tissues.
Image caption:
Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge.
From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7
doi: 10.1186/1471-2172-12-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD1d antibody, clone 1B1 (MCA4700) used for immunofluorescence staining of mouse tissues.
Image caption:
Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.
From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7
doi: 10.1186/1471-2172-12-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD1d antibody, clone 1B1 (MCA4700) used for immunofluorescence staining of mouse tissues.
Image caption:
Intrafollicular location of 56R and AM14 HC Tg MZB cells. Representative spleen sections of B6.Sle2.56R and B6.56R (A, 100X) and B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b mice stained with Moma-1-FITC, CD1d-PE, and IgMa-biotin-SA-PB..
From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7
doi: 10.1186/1471-2172-12-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD1d antibody, clone 1B1 (MCA4700) used for immunofluorescence staining of mouse tissues.
Image caption:
Intrafollicular location of 56R and AM14 HC Tg MZB cells. Representative spleen sections of B6.Sle2.56R and B6.56R and B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b (B, 200X) mice stained with Moma-1-FITC, CD1d-PE, and IgMa-biotin-SA-PB.
From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7
doi: 10.1186/1471-2172-12-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD1d
- Product Type
- Monoclonal Antibody
- Clone
- 1B1
- Isotype
- IgG2b
- Specificity
- CD1d
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD1d antibody, clone 1B1 recognizes mouse CD1d, a glycoprotein expressed on cortical thymocytes, certain T cell leukemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse CD1d cDNA-transfected RMA-S mouse T lymphoma
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/20 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD1d antibody
-
Sun, J. et al. (2010) Deficiency of antigen-presenting cell invariant chain reduces atherosclerosis in mice.
Circulation 122 (8): 808-20. -
Zhou, Z. et al. (2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 12: 7. -
Soh, S.Y. et al. (2016) NKT Cell Hyporesponsiveness Leads to Unrestrained Accumulation of Marginal Zone B Cells in Hypercholesterolemic Apolipoprotein E-Deficient Mice.
J Immunol. 197 (10): 3894-904.
- RRID
- AB_2228873
- UniProt
- P11609
- Entrez Gene
- Cd1d1
- GO Terms
- GO:0005515 protein binding
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0009897 external side of plasma membrane
- GO:0010008 endosome membrane
- GO:0030883 endogenous lipid antigen binding
- View More GO Terms
- GO:0045059 positive thymic T cell selection
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045087 innate immune response
- GO:0045404 positive regulation of interleukin-4 biosynthetic process
- GO:0048007 antigen processing and presentation, exogenous lipid antigen via MHC class Ib
- GO:0051135 positive regulation of NK T cell activation
- GO:0051138 positive regulation of NK T cell differentiation
MCA4700
148866
158172
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