CD199 antibody | 9B1

Rat anti Mouse CD199

Product Type
Monoclonal Antibody
Clone
9B1
Isotype
IgG2a
Specificity
CD199

Product Code Applications Pack Size List Price Your Price Qty
MCA2586GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Rat anti Mouse CD199 antibody, clone 9B1 detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9.

CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.

Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
25 amino acids from the amino terminal of murine CCR9, coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Lewis rat were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD199 antibody

  1. Rivera-nieves, J. et al. (2006) Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis.
    Gastroenterology. 131 (5): 1518-29.
  2. Nagakubo, D. et al. (2007) Expression of CCR9 in HTLV-1+ T cells and ATL cells expressing Tax.
    Int J Cancer. 120 (7): 1591-7.

Further Reading

  1. Uehara, S. et al. (2006) Premature expression of chemokine receptor CCR9 impairs T cell development.
    J Immunol. 176 (1): 75-84.

Synonyms
CCR9
RRID
AB_1604844
UniProt
Q9WUT7
Entrez Gene
Ccr9
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0006935 chemotaxis
GO:0016493 C-C chemokine receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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