CD199 antibody | 9B1
Rat anti Mouse CD199
- Product Type
- Monoclonal Antibody
- Clone
- 9B1
- Isotype
- IgG2a
- Specificity
- CD199
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD199 antibody, clone 9B1 detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9. CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described. Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- 25 amino acids from the amino terminal of murine CCR9, coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Lewis rat were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Rivera-Nieves, J. et al. (2006) Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis.
Gastroenterology. 131 (5): 1518-29.
Further Reading
-
Uehara, S. et al. (2006) Premature expression of chemokine receptor CCR9 impairs T cell development.
J Immunol. 176 (1): 75-84.
- Synonyms
- CCR9
- RRID
- AB_1604844
- UniProt
- Q9WUT7
- Entrez Gene
- Ccr9
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0006935 chemotaxis
- GO:0016493 C-C chemokine receptor activity
MCA2586GA
159777
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