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CD169 antibody | 3D6.112

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 0.1 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 0.2 mg loader
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Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991).

CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014).

The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Purified murine sialoadhesin.
Approx. Protein Concentrations
MCA884GA: IgG concentration 1 mg/ml
MCA884: IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunized AO rat were fused with the cells of the Y3 rat myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/1000
Immunohistology - Frozen 1 1/50 1/100
  1. 1Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD169 antibody

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    EMBO J. 10 (7): 1661-9.
  2. Barnes, Y.C. et al. (1999) Sialylation of the sialic acid binding lectin sialoadhesin regulates its ability to mediate cell adhesion.
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  4. Hughes, E.H. et al. (2003) Generation of Activated Sialoadhesin-Positive Microglia during Retinal Degeneration.
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    J Exp Med. 201: 1615-25.
  7. Krücken, J. et al. (2005) Massive destruction of malaria-parasitized red blood cells despite spleen closure.
    Infect Immun. 73: 6390-8.
  8. Vora, K.A. et al. (2005) Sphingosine 1-phosphate receptor agonist FTY720-phosphate causes marginal zone B cell displacement.
    J Leukoc Biol. 78: 471-80.
  9. View The Latest Product References
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  12. Sancho-Pelluz, J. et al. (2008) Sialoadhesin expression in intact degenerating retinas and following transplantation.
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  14. Taylor, P.R. et al. (2008) Development of a specific system for targeting protein to metallophilic macrophages.
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  15. Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
    Proc Natl Acad Sci U S A. 105: 19571-8.
  16. Peng, Y. et al. (2009) Ly6C(low) monocytes differentiate into dendritic cells and cross-tolerize T cells through PDL-1.
    J Immunol. 182: 2777-85.
  17. Toda, M. et al. (2009) Ligation of tumour-produced mucins to CD22 dramatically impairs splenic marginal zone B-cells.
    Biochem J. 417: 673-83.
  18. Qiu, C.H. et al. (2009) Novel subset of CD8{alpha}+ dendritic cells localized in the marginal zone is responsible for tolerance to cell-associated antigens.
    J Immunol. 182: 4127-36.
  19. Malkiel, S. et al. (2009) The loss and gain of marginal zone and peritoneal B cells is different in response to relapsing fever and Lyme disease Borrelia.
    J Immunol. 182: 498-506.
  20. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
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  21. Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
    J Gen Virol. 90:33-43.
  22. Idoyaga, J. et al. (2009) Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.
    Proc Natl Acad Sci U S A. 106: 1524-9.
  23. Huang, Q.Q. et al. (2010) FLIP: a novel regulator of macrophage differentiation and granulocyte homeostasis.
    Blood. 116: 4968-77.
  24. Barral, P. et al. (2010) CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes.
    Nat Immunol. 11: 303-12.
  25. Albacker, L.A. et al. (2010) TIM-4, a receptor for phosphatidylserine, controls adaptive immunity by regulating the removal of antigen-specific T cells.
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  27. Phillips, R. et al. (2010) Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7.
    PLoS Pathog. 6(3):e1000813.
  28. Hashimoto, D. et al. (2011) Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation.
    J Exp Med. 208: 1069-82.
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    J Immunol. 186: 4967-72.
  30. Lu, M. and Munford, R.S. (2011) The transport and inactivation kinetics of bacterial lipopolysaccharide influence its immunological potencyin vivo.
    J Immunol. 187: 3314-20.
  31. Chow, A. et al. (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche.
    J Exp Med. 208: 261-71.
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    J Mol Med (Berl). 92(9):951-9.
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    Stem Cells. 33 (7): 2294-305.
  38. Cantisani, R. et al. (2015) Vaccine adjuvant MF59 promotes retention of unprocessed antigen in lymph node macrophage compartments and follicular dendritic cells.
    J Immunol. 194 (4): 1717-25.
  39. Gao, L. et al. (2015) Infiltration of circulating myeloid cells through CD95L contributes to neurodegeneration in mice.
    J Exp Med. 212 (4): 469-80.
  40. Park, M.H. et al. (2015) Neuropeptide Y regulates the hematopoietic stem cell microenvironment and prevents nerve injury in the bone marrow.
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  42. Asai, H. et al. (2015) Depletion of microglia and inhibition of exosome synthesis halt tau propagation.
    Nat Neurosci. 18 (11): 1584-93.
  43. Xu, H.C. et al. (2015) Deficiency of the B cell-activating factor receptor results in limited CD169+ macrophage function during viral infection.
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  44. Prokopec, K.E. et al. (2016) Marginal Zone Macrophages Regulate Antigen Transport by B Cells to the Follicle in the Spleen via CD21.
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    PLoS One. 13 (10): e0205172.
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    J Neuroinflammation. 15 (1): 194.
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    Cell Rep. 27 (13): 3799-3807.e3.
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Flow Cytometry


Immunohistology - Frozen

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0006897 endocytosis


152132 154991 163141


155393 160050 1610 164650

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