CD169 antibody | 3D6.112
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884GA) used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Splenic marginal zone macrophages and marginal metallophilic macrophages control L. donovani by an IFN-γ independent mechanism. (A) L. donovani amastigotes are found predominantly in MMM and MZM after intravenous injection (purple, SIGNR1; green CD169; white, L. donovani; blue DAPI). (B) C57BL/6 mice were treated with 0.5mg XMG 1.6 anti-IFN-γ IgG (black bars) or control IgG (open bars) and 2h later infected with L. donovani. At 5h and 24h p.i., the number of parasites per MZ area was determined. (C) 14M1.4 cells (black bars) and RAW 264.7 cells (open bars) were infected with L. donovani (MOI 10:1) and at indicated times p.i., parasite numbers per 100 macrophages were determined by fluorescence microscopy. *, p<0.05, **, p<0.01 between bars indicated as indicated. Scale bar; 200μm.
From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010)
Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7.
PLoS Pathog 6(3): e1000813.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884GA) used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
IRF-7 is induced in MZM and MM in situ. (A–D) Induction of IRF-7 in MMM and MZM. Naïve (A,B) and C57BL/6 mice infected for 24h (C, D) were stained for CD169 (A, C; green) or SIGNR1 (B, D; green), IRF-7 (red) and L. donovani (white). (E) The percentage of IRF-7+ parasite-containing phagosomes was quantified by counting 100 amastigotes/section (n = 3) and calculating the proportion of parasites with IRF-7 accumulation. *, p<0.05 (F) Snap shot from Video S4, showing infected MMM with phagosomal localisation of IRF-7 (CD169+ green, IRF-7, red; LV9, white; DAPI, blue). Scale bars; 50μm.
From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010)
Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7.
PLoS Pathog 6(3): e1000813.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884GA) used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Irf-7−/− MZM and MMM are defective in anti-leishmanial activity. (A–F) Parasite localisation in wild type and Irf-7−/− mice. Spleens from naïve C57BL/6 (A) and Irf-7−/− (B) mice and C57BL/6 (C, E) and Irf-7−/− (D, F) mice infected with L. donovani for 5h or 24h, were stained for CD169 (green), SIGNR1 (red), Leishmania (white) and counterstained with DAPI (blue). Higher magnification inserts show localization of amastigotes (arrowed). (G) Parasite load in the MZ of C57BL/6 mice (open bars) and B6.Irf-7−/− mice (black bars) was determined by counting the number of parasites per MZ area *, p<0.05, **, p<0.01. (H) The number of amastigotes in CD169+ MMM and SIGNR1+ MZM of B6 (open bars) and Irf-7−/− (black bars) mice was determined at 5h and 24h (n = 64 MZ profiles, from 4 mice per time point per strain). Data are presented as fold change at 24h. ***, p<0.001. Scale bars; 100μm.
From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010)
Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7.
PLoS Pathog 6(3): e1000813.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884GA) used for the detection of sialoadhesin im mouse spleen by immunofluorescence.
Image caption:
Specificity of IRF-7 polyclonal antibody. (A, B) 14M1.4 cells (A) and knockdown line KD#1 (B) were infected with L. donovani and stained at 24h for IRF-7. (C,D) L. donovani infected B6 mice (C) and B6.Irf7−/− mice (D) were infected for 5h and then stained for IRF-7. No phagosomal staining of IRF-7 was observed in KD#1 or in B6.Irf7−/− mice, confirming that this antibody does not cross react with Leishmania components.
From: Phillips R, Svensson M, Aziz N, Maroof A, Brown N, et al. (2010)
Innate Killing of Leishmania donovani by Macrophages of the Splenic Marginal Zone Requires IRF-7.
PLoS Pathog 6(3): e1000813.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used for the identification of marginal zone metallophils in mouse spleen sections by immunofluorescence.
Image caption:
Localization of IgG2c+ (red) plasma cells in the spleens of 4 month old w.t. and MFG-E8-/- mice was examined by immunofluorescence staining. Red pulp macrophages and Marginal zone macrophages were visualized by anti-F4/80(green, upper) and anti-CD169 (green, lower), respectively. The images are representative of at least 5 mice in each strain.
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used for the identification of marginal zone metallophilic macrophages in mouse spleen sections by immunofluorescence.
Image caption:
Increased percentages of GC B cell and T follicular helper cells in 4 month old MFG-E8-/- B6 mice.
Spleen sections were stained with anti-CD169 and PNA to visualize GCs (right).
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used for the identification of marginal zone metallophilic macrophages in mouse spleen sections by immunofluorescence.
Image caption:
MFG-E8 deficiency promotes the differentiation of anti-ssRNA specific H564 B cells.
Immunofluorescence staining of the spleen sections from w.t. and MFG-E8-/- H564 mice. Top: CD169 (green), Ki67 (red), id (blue). Note that large numbers of id+ plasma cells (blue) were detected around CD169+ macrophages in MFG-E8-/-H564 mice. Bottom: PNA (red), B220 (blue). The images are representative of at least 5 mice in each strain.
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used to lable marginal zone metallophils in the spleen of mice by imunofluorescence on whole organ mounts following lipid clearance.
Image caption:
3D distribution of the sympathetic architecture in the spleen. The spleen was processed for the whole-tissue co-immunolabeling of TH (green) and CD169 (magenta).
Representative 3D images at 1.26× magnification on the light sheet microscope are shown.
From: Ding X, Wang H, Qian X, Han X, Yang L, Cao Y, Wang Q, Yang J.
Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity.
Cell Rep. 2019 Jun 25;27(13):3799-807.e3.
doi: 10.1016/j.celrep.2019.05.082.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used for the detection of sialoadhesin in mouse spleen sections by immunofluorescence.
Image caption:
Multicolor antibody array for mouse spleen using FDMM. Tissue sections from a wild type spleen (WT), a TNFα-receptor 1 knockout spleen (TNFaR1 KO), and a spleen from a CD11b-DTR mouse (CD11b-DTR) were multi-immunolabeled with antibodies against marginal zone macrophages (anti-CD169, cyan), dendritic cells (anti-CD11c, blue), B cells (anti-B220, red), CD4+ T cells (anti-CD4, green), and CD8+ T cells (anti-CD8, magenta). Nuclei were stained with DAPI (not shown). Arrow in TNFα-receptor 1 knockout spleen indicates weak marginal zone structure (cyan). Arrowhead in CD11b-DTR spleen indicates CD4+ T cells (green) within the B cell follicles. All six channels are presented individually in S6 Fig. Scale bar, 100 μm.
From: Kijani S, Yrlid U, Heyden M, Levin M, Borén J, et al. (2015)
Filter-Dense Multicolor Microscopy.
PLoS ONE 10(3): e0119499.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884F) used for the demonstration of metallophillic macrophages in spleen sections by immunofluorescence.
Image caption:
BST-2 contains LCMV within the splenic marginal zone.
Representative confocal images from naïve, d3 WT, and d3 BST-2 KO mice depict the splenic distribution of CD169+ metallophillic macrophages (green; B) in relation to BST-2 expression (red) (n = 4 mice per group; 2 independent experiments).
From: Urata S, Kenyon E, Nayak D, Cubitt B, Kurosaki Y, Yasuda J, et al. (2018)
BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection.
PLoS Pathog 14(7): e1007172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used for the identification of marginal zone metallophilic macrophages in mouse spleen sections by immunofluorescence.
Image caption:
Increased percentages of GC B cell and T follicular helper cells in 4 month old MFG-E8-/- B6 mice.
MFG-E8 deficiency selectively promotes MZ B cell differentiation of anti-dsDNA specific 56R/Vκ38c B cells.
MFG-E8-/- mice were crossed with 56R anti-dsDNA BCR transgenic mice. The non-autoreactive 56R/Vκ21D and autoreactive 56R/Vκ38c B cells in the spleens of 3 month old female mice were determined by idiotype specific antibodies using Immunofluorescence staining (right). The images are representative of at least 5 mice in each strain.
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA884) used to label murine alveolar macrophages by immunofluorescence.
Image caption:
Immunofluorescence analysis of the lung tissue from K18-hACE2 mice infected by SARS-CoV-2 ancestral strain and Delta variant. Eight-week-old female K18-hACE2 mice were intranasally inoculated with PBS (mock group) or 104 PFU of SARS-CoV-2 ancestral or Delta strain (n = 4 for mock group; n = 6 for infection groups). At 6 dpi, lung tissues were collected and processed for immunofluorescence staining. Lung cryosections were labeled with CD3 (T cells), Gr-1 (neutrophils), CD169 (alveolar macrophages), and laminin (parenchymal tissue). Colocalization and distribution of CD3+ T cells, Gr-1+ neutrophils, and CD169+ alveolar macrophages are shown in the image. Images representative of n = 6 mice per group were acquired by confocal microscopy as a z-stack using a 10× (1.0-NA) objective. Scale bars in panels = 150 μm.
From: Liu X, Mostafavi H, Ng WH, Freitas JR, King NJC, Zaid A, Taylor A, Mahalingam S.
The Delta SARS-CoV-2 Variant of Concern Induces Distinct Pathogenic Patterns of Respiratory Disease in K18-hACE2 Transgenic Mice Compared to the Ancestral Strain from Wuhan.
mBio. 2022 Apr 14:e0068322.
doi: 10.1128/mbio.00683-22.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA3D6.112) used to detect siglec-1 expressing cells in mouse popliteal lymph nodes by immunofluorescence.
Image caption:
Siglec1 is expressed on the macrophages on the floor of the LN SCS and medullary region.
(A) Schematic of the LN macrophage depletion experiment to confirm that Siglec1 was expressed on LN macrophages. (B) Confocal micrograph of popliteal LNs of PBS liposomes and animals that had received clodronate liposome footpad injection 7 days earlier. Clodronate treatment depleted the Siglec1-expressing macrophages, thus no Siglec1-positive macrophages could be seen in the SCS and medullary sinus. Note the complete absence of Siglec1 on Lyve1+ SCS and medullary lymphatic endothelial cells. Representative data from two independent experiments.
From: Singh R, Choi BK.
Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 2019 Dec 24;8:e48916.
doi: 10.7554/eLife.48916.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA3D6.112) used to detect siglec-1 expressing cells in mouse popliteal lymph nodes by immunofluorescence.
Image caption:
Steps of lymph node (LN) metastatic colonization.
B6 mice were implanted with B16-GFP cancer cells in footpads. Representative laser scanning microscopy images of cryosectioned sentinel popliteal LNs (pLNs) on indicated days are shown. (A) Image taken at 14 days post-inoculation; pioneer metastatic cells (green) were evident in the subcapsular sinus (SCS) of pLN. Here, metastatic cell could be seen in close contact with Siglec1+ SCS macrophages (red) in the LN SCS lined by lymphatic endothelial cells (blue). (B) A laser scanning microscope image of early metastatic B16F10 cell (green) in close contact with LN SCS macrophages (Siglec1, red; nucleus, blue) and its 3D reconstructed image.
From: Singh R, Choi BK.
Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 2019 Dec 24;8:e48916.
doi: 10.7554/eLife.48916.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA3D6.112) used to detect siglec-1 expressing cells in mouse popliteal lymph nodes by immunofluorescence.
Image caption:
Steps of lymph node (LN) metastatic colonization.
B6 mice were implanted with B16-GFP cancer cells in footpads. Representative laser scanning microscopy images of cryosectioned sentinel popliteal LNs (pLNs) on indicated days are shown. (A) Image taken at 14 days post-inoculation; pioneer metastatic cells (green) were evident in the subcapsular sinus (SCS) of pLN. Here, metastatic cell could be seen in close contact with Siglec1
From: Singh R, Choi BK.
Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 2019 Dec 24;8:e48916.
doi: 10.7554/eLife.48916.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA3D6.112) used to detect siglec-1 expressing cells in mouse popliteal lymph nodes by immunofluorescence.
Image caption:
4T1 mouse breast cancer cells use LN SCS macrophages to find footholds during LN metastasis.
(A) Schematic of experiment. EGFP-expressing 4T1 cells were implanted into BALB/c mouse footpads and popliteal LNs were isolated from day 14 for confocal imaging. The procedure was the same as described in Figure 1—figure supplement 1A. (B) GFP+ 4T1 cells (green) can be seen in close contact with LN SCS macrophages (red) at day 14. A growing LN metastatic colony of 4T1-GFP cells can be seen at day 18 growing in close contact with SCS macrophages. GFP signals in 4T1-GFP cells were amplified using anti-GFP antibody. Representative images from two independent experiments.
From: Singh R, Choi BK.
Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 2019 Dec 24;8:e48916.
doi: 10.7554/eLife.48916.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD169 antibody, clone 3D6.112 (MCA3D6.112) used to detect siglec-1 expressing cells in mouse popliteal lymph nodes by immunofluorescence.
Image caption:
α−2,3-linked sialylation and melanoma LN metastasis.
(F) Morphological features of early metastatic foci (day 17) in the LN SCS of mice injected with WT or St3gal3 KO B16-GFP (green, B16-GFP; red, SCS macrophages). (G) Representative confocal images of Ki67+ (white) nuclei in metastatic foci formed by WT or St3gal3 KO B16-GFP cells in the LN SCS. (I) Representative confocal image of apoptosis in WT and St3gal3 KO B16-GFP cells during the early stages of LN metastatic colonization measured by cleaved caspase-3+ (white) staining.
From: Singh R, Choi BK.
Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 2019 Dec 24;8:e48916.
doi: 10.7554/eLife.48916.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C IF ResetRat anti Mouse CD169
- Product Type
- Monoclonal Antibody
- Clone
- 3D6.112
- Isotype
- IgG2a
- Specificity
- CD169
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Purified murine sialoadhesin.
- Approx. Protein Concentrations
- Pack Size: 0.2 mgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/1000 |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/50 | 1/100 |
- 1Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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References for CD169 antibody
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Sci Rep. 6: 22143. -
Farrell, H.E. et al. (2016) Type 1 Interferons and NK Cells Limit Murine Cytomegalovirus Escape from the Lymph Node Subcapsular Sinus.
PLoS Pathog. 12 (12): e1006069. -
Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
Cardiovasc Res. 114 (3): 456-67. -
Urata, S. et al. (2018) BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection.
PLoS Pathog. 14 (7): e1007172. -
Peng, Y. (2018) B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS One. 13 (10): e0205172. -
Groh, J. et al. (2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
J Neuroinflammation. 15 (1): 194. -
Tay, M.H.D. et al. (2019) Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
Front Immunol. 10: 575. -
Ding, X. et al. (2019) Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity.
Cell Rep. 27 (13): 3799-3807.e3. -
Singh, R. & Choi, B.K. (2019) Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
Elife. 8: e48916. -
Liu, X. et al. (2022) The Delta SARS-CoV-2 Variant of Concern Induces Distinct Pathogenic Patterns of Respiratory Disease in K18-hACE2 Transgenic Mice Compared to the Ancestral Strain from Wuhan.
mBio. : e0068322.
- Synonyms
- Sialoadhesin
- RRID
- AB_322416
- UniProt
- Q62230
- Entrez Gene
- Siglec1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005529 sugar binding
- GO:0006897 endocytosis
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