CD169 antibody | 3D6.112

Rat anti Mouse CD169

Product Type
Monoclonal Antibody
Clone
3D6.112
Isotype
IgG2a
Specificity
CD169

Product Code Applications Pack Size List Price Your Price Qty
MCA884
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 0.2 mg loader
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loader
MCA884GA
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SDS Safety Datasheet SDS
C * F IF 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991).

CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014).

The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified murine sialoadhesin.
Approx. Protein Concentrations
Pack Size: 0.2 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/1000
Immunofluorescence
Immunohistology - Frozen 1 1/50 1/100
  1. 1Bio-Rad recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD169 antibody

  1. Kaisho, T. et al. (2001) IkappaB kinase alpha is essential for mature B cell development and function.
    J Exp Med. 193: 417-26.
  2. Hughes, E.H. et al. (2003) Generation of Activated Sialoadhesin-Positive Microglia during Retinal Degeneration.
    Invest Ophthalmol Vis Sci. 44: 2229-34.
  3. Notley, C.A. et al. (2011) Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    J Immunol. 186: 4967-72.
  4. Ravishankar, B. et al. (2012) Tolerance to apoptotic cells is regulated by indoleamine 2,3-dioxygenase.
    Proc Natl Acad Sci U S A. 109: 3909-14.
  5. Toda, M. et al. (2009) Ligation of tumour-produced mucins to CD22 dramatically impairs splenic marginal zone B-cells.
    Biochem J. 417: 673-83.
  6. Peng, Y. et al. (2009) Ly6C(low) monocytes differentiate into dendritic cells and cross-tolerize T cells through PDL-1.
    J Immunol. 182: 2777-85.
  7. Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
    J Leukoc Biol. 76: 86-94.
  8. Malkiel, S. et al. (2009) The loss and gain of marginal zone and peritoneal B cells is different in response to relapsing fever and Lyme disease Borrelia.
    J Immunol. 182: 498-506.
  9. View The Latest Product References
  10. Qiu, C.H. et al. (2009) Novel subset of CD8{alpha}+ dendritic cells localized in the marginal zone is responsible for tolerance to cell-associated antigens.
    J Immunol. 182: 4127-36.
  11. Miyake, Y. et al. (2007) Protective role of macrophages in noninflammatory lung injury caused by selective ablation of alveolar epithelial type II Cells.
    J Immunol. 178: 5001-9.
  12. Pope, J.G. et al. (1998) Characterization of and functional antigen presentation by central nervous system mononuclear cells from mice infected with Theiler's murine encephalomyelitis virus.
    J Virol. 72: 7762-71.
  13. Cunningham, C. et al. (2005) Comparison of inflammatory and acute-phase responses in the brain and peripheral organs of the ME7 model of prion disease.
    J Virol. 79: 5174-84.
  14. Vora, K.A. et al. (2005) Sphingosine 1-phosphate receptor agonist FTY720-phosphate causes marginal zone B cell displacement.
    J Leukoc Biol. 78: 471-80.
  15. Crocker, P.R. et al. (1991) Purification and properties of sialoadhesin, a sialic acid-binding receptor of murine tissue macrophages.
    EMBO J. 10 (7): 1661-9.
  16. Sancho-Pelluz, J. et al. (2008) Sialoadhesin expression in intact degenerating retinas and following transplantation.
    Invest Ophthalmol Vis Sci. 49: 5602-10.
  17. Barral, P. et al. (2010) CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes.
    Nat Immunol. 11: 303-12.
  18. Chtanova, T. et al. (2008) Dynamics of neutrophil migration in lymph nodes during infection.
    Immunity. 29: 487-96.
  19. Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
    J Gen Virol. 90:33-43.
  20. Iannacone M (2010) Subcapsular sinus macrophages prevent CNS invasion on peripheral infection with a neurotropic virus.
    Nature. 465: 1079-83.
  21. Idoyaga, J. et al. (2009) Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.
    Proc Natl Acad Sci U S A. 106: 1524-9.
  22. Taylor, P.R. et al. (2008) Development of a specific system for targeting protein to metallophilic macrophages.
    Proc Natl Acad Sci U S A. 101: 1963-8.
  23. Chang, Y.C. et al. (2014) Role of macrophage sialoadhesin in host defense against the sialylated pathogen group B Streptococcus.
    J Mol Med (Berl). 92(9):951-9.
  24. Lin, H.H. et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
    J Exp Med. 201: 1615-25.
  25. Phillips, R. et al. (2010) Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7.
    PLoS Pathog. 6(3):e1000813.
  26. Hashimoto, D. et al. (2011) Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation.
    J Exp Med. 208: 1069-82.
  27. Anderson, K.L. et al. (1999) PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation.
    Blood. 94: 2310-8.
  28. Albacker, L.A. et al. (2010) TIM-4, a receptor for phosphatidylserine, controls adaptive immunity by regulating the removal of antigen-specific T cells.
    J Immunol. 185: 6839-49.
  29. Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
    Proc Natl Acad Sci U S A. 105: 19571-8.
  30. Chow, A. et al. (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche.
    J Exp Med. 208: 261-71.
  31. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
    J Immunol. 182: 1278-86.
  32. Hemmi, H. et al. (2012) Treml4, an Ig superfamily member, mediates presentation of several antigens to T cells in vivo, including protective immunity to HER2 protein.
    J Immunol. 188: 1147-55.
  33. Huang, Q.Q. et al. (2010) FLIP: a novel regulator of macrophage differentiation and granulocyte homeostasis.
    Blood. 116: 4968-77.
  34. Lu, M. and Munford, R.S. (2011) The transport and inactivation kinetics of bacterial lipopolysaccharide influence its immunological potencyin vivo.
    J Immunol. 187: 3314-20.
  35. Krücken, J. et al. (2005) Massive destruction of malaria-parasitized red blood cells despite spleen closure.
    Infect Immun. 73: 6390-8.
  36. Vagaja, N.N. et al. (2012) Changes in murine hyalocytes are valuable early indicators of ocular disease.
    Invest Ophthalmol Vis Sci. 53: 1445-51.
  37. Barnes, Y.C. et al. (1999) Sialylation of the sialic acid binding lectin sialoadhesin regulates its ability to mediate cell adhesion.
    Blood. 93: 1245-52.
  38. Chen, W.C. et al. (2012) Antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/CD169.
    PLoS One. 7e39039.
  39. Mansour, A. et al. (2012) Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow.
    J Exp Med. 209: 537-49.
  40. Park, M.H. et al. (2015) Neuropeptide Y regulates the hematopoietic stem cell microenvironment and prevents nerve injury in the bone marrow.
    EMBO J. 34 (12): 1648-60.
  41. Asai, H. et al. (2015) Depletion of microglia and inhibition of exosome synthesis halt tau propagation.
    Nat Neurosci. 18 (11): 1584-93.
  42. Farrell, H.E. et al. (2015) Lymph Node Macrophages Restrict Murine Cytomegalovirus Dissemination.
    J Virol. 89 (14): 7147-58.
  43. Xu, H.C. et al. (2015) Deficiency of the B cell-activating factor receptor results in limited CD169+ macrophage function during viral infection.
    J Virol. 89 (9): 4748-59.
  44. Gao, L. et al. (2015) Infiltration of circulating myeloid cells through CD95L contributes to neurodegeneration in mice.
    J Exp Med. 212 (4): 469-80.
  45. McCabe, A. et al. (2015) Macrophage-Lineage Cells Negatively Regulate the Hematopoietic Stem Cell Pool in Response to Interferon Gamma at Steady State and During Infection.
    Stem Cells. 33 (7): 2294-305.
  46. Cantisani, R. et al. (2015) Vaccine adjuvant MF59 promotes retention of unprocessed antigen in lymph node macrophage compartments and follicular dendritic cells.
    J Immunol. 194 (4): 1717-25.
  47. Prokopec, K.E. et al. (2016) Marginal Zone Macrophages Regulate Antigen Transport by B Cells to the Follicle in the Spleen via CD21.
    J Immunol. 197 (6): 2063-8.
  48. Li, Z. et al. (2016) The Macrophage-depleting Agent Clodronate Promotes Durable Hematopoietic Chimerism and Donor-specific Skin Allograft Tolerance in Mice.
    Sci Rep. 6: 22143.
  49. Farrell, H.E. et al. (2016) Type 1 Interferons and NK Cells Limit Murine Cytomegalovirus Escape from the Lymph Node Subcapsular Sinus.
    PLoS Pathog. 12 (12): e1006069.
  50. Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
    Cardiovasc Res. 114 (3): 456-67.
  51. Urata, S. et al. (2018) BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection.
    PLoS Pathog. 14 (7): e1007172.
  52. Peng, Y. (2018) B cell responses to apoptotic cells in MFG-E8-/- mice.
    PLoS One. 13 (10): e0205172.
  53. Tay, M.H.D. et al. (2019) Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
    Front Immunol. 10: 575.
  54. Ding, X. et al. (2019) Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity.
    Cell Rep. 27 (13): 3799-3807.e3.
  55. Groh, J. et al. (2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
    J Neuroinflammation. 15 (1): 194.
  56. Liu, X. et al. (2022) The Delta SARS-CoV-2 Variant of Concern Induces Distinct Pathogenic Patterns of Respiratory Disease in K18-hACE2 Transgenic Mice Compared to the Ancestral Strain from Wuhan.
    mBio. : e0068322.
  57. Singh, R. & Choi, B.K. (2019) Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
    Elife. 8: e48916. [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Synonyms
Sialoadhesin
RRID
AB_322416
UniProt
Q62230
Entrez Gene
Siglec1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0006897 endocytosis
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