CD16/CD32 antibody | FCR4G8
Rat anti Mouse CD16/CD32 antibody, clone FCRG8 (MCA2305) used for evaluation of FcRII/III expression in murine brain by immunohistochemistry.
Image caption:
Macrophage and microglia activation in the brain after intracerebral injection of OVA. OVA-immunized mice or control non-immunized mice received a unilateral injection of OVA into the striatum and were assessed for presence of macrophage and microglia activation. Data shows CD11b, MHCII, F4/80 and FcγRII/III immunoreactivity after 24 h, 3 days or 7 days in OVA-immunized mice or non-immunized mice. As a positive control for these well-characterized antibodies spleen tissue from a naïve mouse was used. The number of DAB-positive pixels (cells and their processes) in OVA-immunized (black bars) and non-immunized mice (white bars) was quantified as described in “Materials and methods”. Statistical analysis: One-way ANOVA, Dunnett's post-test *p <0.05. Data represents the mean of n = 3–5 per treatment and time point. The experiment was performed twice independently with comparable results
From: Teeling JL, Carare RO, Glennie MJ, Perry VH.
Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
Acta Neuropathol. 2012 Oct;124(4):479-90.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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F C ResetRat anti Mouse CD16/CD32
- Product Type
- Monoclonal Antibody
- Clone
- FCR4G8
- Isotype
- IgG2b
- Specificity
- CD16/CD32
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- PU5 1.8 IOE7 Balb/c mouse cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD16/CD32 antibody
-
da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
Am J Pathol. 177 (3): 1534-48. -
Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186: 3441-51. -
Jones, D.T. et al. (2013) Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice.
Dis Model Mech. 6: 115-24. -
Kapellos, T.S. et al. (2016) A Novel Real Time Imaging Platform to Quantify Macrophage Phagocytosis.
Biochem Pharmacol. Jul 27. pii: S0006-2952(16)30176-9. [Epub ahead of print] -
Lunnon, K. et al. (2011) Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.
J Immunol. 186 (12): 7215-24. -
Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
Acta Neuropathol. 124 (4): 479-90. -
Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
Invest Ophthalmol Vis Sci. 55 (1): 247-58. -
Hart, A.D. et al. (2012) Age related changes in microglial phenotype vary between CNS regions: grey versus white matter differences.
Brain Behav Immun. 26 (5): 754-65.
View The Latest Product References
-
Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186 (6): 3441-51. -
Hart, R. & Greaves, D.R. (2010) Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5.
J Immunol. 185 (6): 3728-39. -
Ahmed, N. et al. (2022) Blood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation.
J Exp Med. 219 (1): e20202490.
- Synonyms
- Mouse Seroblock FcR
- RRID
- AB_2103731
- UniProt
- P08508
- P08101
- Entrez Gene
- Fcgr2b
- Fcgr3
- GO Terms
- GO:0005886 plasma membrane
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001805 positive regulation of type III hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0001820 serotonin secretion
- GO:0016021 integral to membrane
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- View More GO Terms
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0019770 IgG receptor activity
- GO:0030593 neutrophil chemotaxis
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
- GO:0045576 mast cell activation
- GO:0050766 positive regulation of phagocytosis
View more products with CD16 specificity
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