CD16 antibody | AT154-2

Rat anti Mouse CD16

Product Type
Monoclonal Antibody
Clone
AT154-2
Isotype
IgG2b
Specificity
CD16

Product Code Applications Pack Size List Price Your Price Qty
MCA5998
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.

In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
HEK293F cell expressing CD16 and gamma chain
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Immunohistology - Frozen 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD16 antibody

  1. White AL et al. (2011) Interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 monoclonal antibody.
    J Immunol. 187 (4): 1754-63.
  2. Tutt, A.L. et al. (2015) Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors.
    J Immunol. 195 (11): 5503-16.

Synonyms
FcRIII
UniProt
P08508
Entrez Gene
Fcgr3
GO Terms
GO:0005886 plasma membrane
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001805 positive regulation of type III hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0001820 serotonin secretion
GO:0016021 integral to membrane
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0007166 cell surface receptor linked signaling pathway
GO:0019770 IgG receptor activity
GO:0030593 neutrophil chemotaxis
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0045576 mast cell activation
GO:0050766 positive regulation of phagocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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