CD150 antibody | 9D1

Rat anti Mouse CD150

Product Type
Monoclonal Antibody
Clone
9D1
Isotype
IgG1
Specificity
CD150

Product Code Applications Pack Size List Price Your Price Qty
MCA2274GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD150 antibody, clone 9D1 recognizes murine CD150, also known as signaling lymphocyte activation molecule (SLAM) . Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and is upregulated on activated T-cells and stimulated macrophages. The extracellular domain of CD150 is the receptor for the measles virus (Erlenhoefer et al. 2001) and acts as a co-activator on T-cells and B-cells.

Rat anti Mouse CD150 antibody, clone 9D1 has functional activity (Howie et al. 2002).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO cells stably transfected with mouse CD150
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of a myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD150 antibody

  1. Howie, D. et al. (2002) The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production.
    Blood. 100 (8): 2899-907.
  2. Desponts, C. et al. (2006) SHIP deficiency enhances HSC proliferation and survival but compromises homing and repopulation.
    Blood. 107: 4338-45.
  3. Almolda, B. et al. (2015) Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10.
    Brain Behav Immun. 45: 80-97.
  4. Villacampa, N. et al. (2015) Astrocyte-targeted production of IL-10 induces changes in microglial reactivity and reduces motor neuron death after facial nerve axotomy.
    Glia. 63 (7): 1166-84.

Synonyms
SLAM
RRID
AB_324921
UniProt
Q9QUM4
Entrez Gene
Slamf1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0046649 lymphocyte activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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