CD150 antibody | 9D1
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F ResetRat anti Mouse CD150
- Product Type
- Monoclonal Antibody
- Clone
- 9D1
- Isotype
- IgG1
- Specificity
- CD150
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD150 antibody, clone 9D1 recognizes murine CD150, also known as signaling lymphocyte activation molecule (SLAM) . Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and is upregulated on activated T-cells and stimulated macrophages. The extracellular domain of CD150 is the receptor for the measles virus (Erlenhoefer et al. 2001) and acts as a co-activator on T-cells and B-cells. Rat anti Mouse CD150 antibody, clone 9D1 has functional activity (Howie et al. 2002). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CHO cells stably transfected with mouse CD150
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of a myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
References for CD150 antibody
-
Howie, D. et al. (2002) The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production.
Blood. 100 (8): 2899-907. -
Desponts, C. et al. (2006) SHIP deficiency enhances HSC proliferation and survival but compromises homing and repopulation.
Blood. 107: 4338-45. -
Almolda, B. et al. (2015) Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10.
Brain Behav Immun. 45: 80-97. -
Villacampa, N. et al. (2015) Astrocyte-targeted production of IL-10 induces changes in microglial reactivity and reduces motor neuron death after facial nerve axotomy.
Glia. 63 (7): 1166-84.
- Synonyms
- SLAM
- RRID
- AB_324921
- UniProt
- Q9QUM4
- Entrez Gene
- Slamf1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- GO:0046649 lymphocyte activation
View more products with CD150 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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