CD147 antibody | OX114

Rat anti Mouse CD147

Product Type
Monoclonal Antibody
Clone
OX114
Isotype
IgG1
Specificity
CD147

Product Code Applications Pack Size List Price Your Price Qty
MCA2283
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.25 mg loader
List Price Your Price
loader
MCA2283GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD147 antibody, clone OX114 recognizes mouse CD147 (also known as basigin). CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.

Rat anti Mouse CD147 antibody, clone OX114 recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains.

Rat anti Mouse CD147 antibody, clone OX114 is routinely tested in flow cytometry on mouse splenocytes. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Fusion protein mCD147dO-2rCD4d3 +4
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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References for CD147 antibody

  1. Hanna, S.M. et al. (2003) A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically.
    BMC Biochemistry 4:17.
  2. Agrawal, S.M. et al. (2011) A Novel Regulator of Leukocyte Transmigration into the CNS in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis.
    J Neurosci. 31: 669-77.
  3. Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
    Gene Ther. 18 (6): 613-21.
  4. Kaushik, D.K. et al. (2019) Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis.
    J Clin Invest. 129 (8): 3277-92.

Flow Cytometry

Immunofluorescence

Western Blotting

Synonyms
Neurothelin
RRID
AB_566521
UniProt
P18572
Entrez Gene
Bsg
GO Terms
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0042470 melanosome
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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