CD137 antibody | LOB12

Rat anti Mouse CD137

Product Type
Monoclonal Antibody
Clone
LOB12
Isotype
IgG2a
Specificity
CD137

Product Code Applications Pack Size List Price Your Price Qty
MCA1951
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD137 antibody, clone LOB12 recognizes murine CD137 also known as Tumor necrosis factor receptor superfamily member 9, T-cell antigen 4-1BB or 4-1BB ligand receptor. CD137 is a 256 amino acid ~28 kDa single pass type I transmembrane glycoprotein.

CD137 is expressed by activated T lymphocytes, but not by resting cells.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Murine CD137 human Fc fusion protein
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Rat IgG2a Negative Control

Source Reference

  1. Taraban, V.Y. et al. (2002) Expression and costimulatory effects of the TNF receptor superfamily members CD134 (OX40) and CD137 (4-1BB), and their role in the generation of anti-tumor immune responses.
    Eur J Immunol. 32 (12): 3617-27.

RRID
AB_322290
UniProt
P20334
Entrez Gene
Tnfrsf9
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0042127 regulation of cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products