Rat anti Mouse CD107a antibody, clone 1D4B recognises murine CD107a, otherwise known as Lysosome-associated membrane glycoprotein 1 or LAMP-1. CD107a is a glycoprotein responsible for presenting carbohydrate ligands to selectins. It may also play a role in tumour metastasis. It is expressed ubiquitously, with higher levels in spleen, liver and kidney. CD107a may be used as a marker for lysosomal storage disorders.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Mouse 3T3 embryonic fibroblastoid cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the non-secreting murine P3-X63Ag8 myeloma.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
integral to membrane
external side of plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD107a antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
Chen, J.W. et al. (1985) Identification of two lysosomal membrane glycoproteins.
J Cell Biol. 101 (1): 85-95.
References for CD107a antibody
Andrejewski, N. et al. (1999) Normal lysosomal morphology and function in LAMP-1-deficient mice.
J Biol Chem. 274 (18): 12692-701.
Winder, A.J. et al. (1993) The mouse brown (b) locus protein has dopachrome tautomerase activity and is located in lysosomes in transfected fibroblasts.
J Cell Sci. 106 ( Pt 1): 153-66.
Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
Glia. 66 (5): 1034-52.
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