CD106 antibody | MVCAM A (429)

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Rat anti Mouse CD106:Alexa Fluor® 488

Rat anti Mouse CD106

Rat anti Mouse CD106:RPE

Product Type
Monoclonal Antibody
Clone
MVCAM A (429)
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2297A488T F 25 Tests/0.25ml
MCA2297A488 F 100 Tests/1ml
MCA2297GA C F IF 0.1 mg
MCA2297 C F IF 0.25 mg
MCA2297PET F 25 Tests
MCA2297PE F 100 Tests
Rat anti Mouse CD106 antibody, clone MVCAM A (429) recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29).

Rat anti Mouse CD106 antibody, clone MVCAM A (429) is reported to partially block VCAM-1 mediated functions (Kinashi and Springer 1994).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Stromal cell line PA6.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P29533 Related reagents
Entrez Gene
Vcam1 Related reagents
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0016021 integral to membrane
GO:0007507 heart development
GO:0031225 anchored to membrane
GO:0044419 interspecies interaction between organisms
GO:0060669 embryonic placenta morphogenesis
GO:0060710 chorio-allantoic fusion
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD106 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/100 Pack Size: 0.1 mg
1/10 Pack Size: 0.25 mg
1/200 Pack Size: 0.1 mg
1/50 Pack Size: 0.25 mg
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488 MCA1212A488 100 Tests/1ml F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for CD106 antibody

  1. Kinashi, T. and Springer, T.A. (1994) Adhesion molecules in hematopoietic cells.
    Blood Cells. 20:25-44.
  2. Wormley, F.L. Jr et al. (2001) Cell adhesion molecule and lymphocyte activation marker expression during experimental vaginal candidiasis.
    Infect Immun. 69 (8): 5072-9.
  3. Woo, J.M. et al. (2010) Treatment with apolipoprotein A-1 mimetic peptide reduces lupus-like manifestations in a murine lupus model of accelerated atherosclerosis.
    Arthritis Res Ther. 12(3):R93.
  4. Hall, L.J. et al. (2010) Probing local innate immune responses after mucosal immunisation.
    J Immune Based Ther Vaccines. 8: 5.
  5. Winnik S et al. (2011) Dietary α-linolenic acid diminishes experimental atherogenesis and restricts T cell-driven inflammation.
    Eur Heart J. 32 (20): 2573-84.
  6. Hamada, T. et al. (2009) Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
    Am J Pathol. 174: 2265-77.
  7. Hamada, T. et al. (2008) Metalloproteinase-9 deficiency protects against hepatic ischemia/reperfusion injury.
    Hepatology. 47: 186-98.
  8. Baumer, Y. et al. (2011) Telomerase-based immortalization modifies the angiogenic/inflammatory responses of human coronary artery endothelial cells.
    Exp Biol Med (Maywood). 236: 692-700.
  9. Lau,. H.Y. and Bhatia, M. (2007) Effect of CP-96,345 on the expression of adhesion molecules in acute pancreatitis in mice.
    Am J Physiol Gastrointest Liver Physiol. 292: G1283-92.
  10. Kumpers, P. et al. (2011) The synthetic tie2 agonist peptide vasculotide protects against vascular leakage and reduces mortality in murine abdominal sepsis.
    Crit Care. 15 (5): R261.
  11. Püntener, U. et al. (2012) Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.
    J Neuroinflammation. 9: 146.
  12. David, S. et al. (2011) Acute administration of recombinant Angiopoietin-1 ameliorates multiple-organ dysfunction syndrome and improves survival in murine sepsis.
    Cytokine. 55 (2): 251-9.
  13. Kuriyama, N. et al. (2011) Tenascin-C: a novel mediator of hepatic ischemia and reperfusion injury.
    Hepatology. 54 (6): 2125-36.
  14. Braach, N. et al. (2014) RAGE controls activation and anti-inflammatory signalling of protein C.
    PLoS One. 9 (2): e89422.
  15. Li, M. et al. (2014) The indoleamine 2,3-dioxygenase pathway controls complement-dependent enhancement of chemo-radiation therapy against murine glioblastoma.
    J Immunother Cancer. 2: 21.