CD106 antibody | MVCAM A (429)
Rat anti Mouse CD106 antibody. clone MVCAM A (MCA2297) used to evaluate VCAM-1 expression in the murine thalamus by immunohistochemistry on cryostat sections.
Image caption:
Extract from figure showing VCAM-1 expression.
Kinetics of immune marker expression after SL3261 infection. Immune markers of vascular endothelial cells and microglia/macrophages (MHCI (A-D), MHCII (E-H), ICAM-1 (I-L), VCAM-1 (M-P), CD11b (Q-T), and CD68 (U-X)) at 1d (B, F, J, N, R, V), 7d (C, G, K, O, S, W), and 21d (D, H, L, P, T, X) after a systemic challenge with Salmonella typhimurium SL3261 compared to naïve non-infected mice (A, E, I, M, Q, U). Representative images from the dorsal lateral geniculate nucleus of the thalamus are shown. Phenotype analysis was carried out with n - 3 mice per time point. Scale bar - 50 μm.
From: Püntener U, Booth SG, Perry VH, Teeling JL.
Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.
J Neuroinflammation. 2012 Jun 27;9:146.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD106 antibody, clone MVCAM A (MCA2297) used for the evaluation of VCAM-1 staining in mouse kidney sections by immunofluorescence.
Image caption:
Extract from Fig 2 to show VCAM-1 staining: Vasculotide (VT) ameliorates acute kidney injury.
Mice were pretreated with VT (200 ng intraperitoneally) or phosphate-buffered saline at 16 hours and 1 hour prior to cecal ligation and puncture (CLP) or sham surgery, respectively. Kidney tissues were harvested at 18 hours after CLP or sham treatment. (A) Immunofluorescence study of intercellular adhesion molecule-1 (ICAM-1) and (B) vascular cell adhesion molecule-1 (VCAM-1) protein expression (red) among specific sections within the renal vasculature (that is, glomeruli [400 ×] arteries and peritubular capillaries [200 ×]). (C) Immunofluorescence study of infiltrating neutrophils (red) in Gr-1-stained sections (200 ×). Bar charts show the results of semiquantitative scoring results (see Materials and methods). (D) Bar charts of serum levels of serum creatinine and urea. Data are expressed as mean ± standard error of the mean (n = 7 to 10 mice per group). **P <0.01 versus sham. †P < 0.05; ‡P < 0.01 versus CLP without VT. HPF, high-power field.
From: Kumpers P, Gueler F, David S, Slyke PV, Dumont DJ, Park JK, Bockmeyer CL, Parikh SM, Pavenstadt H, Haller H, Shushakova N.
The synthetic tie2 agonist peptide vasculotide protects against vascular leakage and reduces mortality in murine abdominal sepsis.
Crit Care. 2011;15(5):R261.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD106 antibody, clone MVCAM A (MCA2297) used to demonstrate VCAM-1 expression in cremaster muscle wholemounts by immunohistochemistry.
Image caption:
Extract from Fig 7 to indicate VCAM-1 immunostaining: Effect of aPC on endothelial ICAM-1 and VCAM-1 expression in vivo.
In vivo endothelial immunostaining in WT (left side) and RAGE−/− (right side) mice after treatment with aPC (24 μg/kg/h, 3 hours; C & D and G & H) or saline (A & B and E & F) was carried out to receive further information about ICAM-1 (A–D) and VCAM-1 (E–H) expression in TNFα-stimulated cremaster muscle venules. Representative micrographs are shown for at least three mice per group. Reference bar for (A–H) is shown in (A) and represents 50 μm.
From: Braach N, Frommhold D, Buschmann K, Pflaum J, Koch L, Hudalla H, et al. (2014)
RAGE Controls Activation and Anti-Inflammatory Signalling of Protein C.
PLoS ONE 9(2): e89422.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD106 antibody, clone MVCAM A (MCA2297) used for the demonstration of VCAM-1 staining in tumor tissue by immunofluorescence on cryostat sections.
Image caption:
IDO-blockade drives vascular activation after chemotherapy and tumor necrosis after chemo-radiation therapy. A, GL261 tumors were grown in WT host mice treated with TMZ (100 mg/kg, i.p.) and with or without IDO-blockade using 1MT (4 mg/mL in drinking water). Tumors were harvested 5 days after chemotherapy (18 days after implantation) and frozen for immunohistochemical analysis of vascular cell adhesion molecule-1 (VCAM-1, red) on endothelial cells (CD31, green). Nuclei were counterstained with Hoechst (blue). Representative photomicrographs are shown of at least 3 mice per group, from at least 3 independent experiments. Original magnification, ×400; Scale bars, 25 μm.
From: Li M, Bolduc AR, Hoda MN, Gamble DN, Dolisca SB, Bolduc AK, Hoang K, Ashley C, McCall D, Rojiani AM, Maria BL, Rixe O, MacDonald TJ, Heeger PS, Mellor AL, Munn DH, Johnson TS.
The indoleamine 2,3-dioxygenase pathway controls complement-dependent enhancement of chemo-radiation therapy against murine glioblastoma.
J Immunother Cancer. 2014 Jul 7;2:21.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
R-phycoetythrin conjugated Rat anti Mouse CD106 antibody, clone MVCAM A (429) (MCA2297PE) used to characterize stromal cells by flow cytometry.
Image caption:
Gating strategy for stromal cells analysis. From living cells, CD45- cells were gated and subpopulations were identified using podoplanin+ (gp38), CD21/CD35+ (FDC), and Lyve-1+ (lymphatic endothelial cells) cells. Surface marker MHCII, CD106, and CD54 were gated from gp38+ cells or CD21/CD35+ cells.
From: Basic M, Peppermüller PP, Bolsega S, Bleich A, Bornemann M, Bode U, Buettner M.
Lymph Node Stromal Cells From Different Draining Areas Distinctly Regulate the Development of Chronic Intestinal Inflammation. Front Immunol. 2021 Feb 16;11:549473.
doi: 10.3389/fimmu.2020.549473.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
P IF C F ResetRat anti Mouse CD106
- Product Type
- Monoclonal Antibody
- Clone
- MVCAM A (429)
- Isotype
- IgG2a
- Specificity
- CD106
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD106 antibody, clone MVCAM A (429) recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29). Rat anti Mouse CD106 antibody, clone MVCAM A (429) is reported to partially block VCAM-1 mediated functions (Kinashi and Springer 1994). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Stromal cell line PA6.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Kinashi, T. and Springer, T.A. (1994) Adhesion molecules in hematopoietic cells.
Blood Cells. 20:25-44.
References for CD106 antibody
-
Lau,. H.Y. and Bhatia, M. (2007) Effect of CP-96,345 on the expression of adhesion molecules in acute pancreatitis in mice.
Am J Physiol Gastrointest Liver Physiol. 292: G1283-92. -
Hamada, T. et al. (2008) Metalloproteinase-9 deficiency protects against hepatic ischemia/reperfusion injury.
Hepatology. 47: 186-98. -
Hamada, T. et al. (2009) Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
Am J Pathol. 174: 2265-77. -
Hall, L.J. et al. (2010) Probing local innate immune responses after mucosal immunisation.
J Immune Based Ther Vaccines. 8: 5. -
Woo, J.M. et al. (2010) Treatment with apolipoprotein A-1 mimetic peptide reduces lupus-like manifestations in a murine lupus model of accelerated atherosclerosis.
Arthritis Res Ther. 12(3):R93. -
Chen, Q. et al. (2011) Macrophage binding to receptor VCAM-1 transmits survival signals in breast cancer cells that invade the lungs.
Cancer Cell. 20 (4): 538-49. -
Kuriyama, N. et al. (2011) Tenascin-C: a novel mediator of hepatic ischemia and reperfusion injury.
Hepatology. 54 (6): 2125-36. -
David, S. et al. (2011) Acute administration of recombinant Angiopoietin-1 ameliorates multiple-organ dysfunction syndrome and improves survival in murine sepsis.
Cytokine. 55 (2): 251-9.
View The Latest Product References
-
Kümpers, P. et al. (2011) The synthetic tie2 agonist peptide vasculotide protects against vascular leakage and reduces mortality in murine abdominal sepsis.
Crit Care. 15 (5): R261. -
Winnik S et al. (2011) Dietary α-linolenic acid diminishes experimental atherogenesis and restricts T cell-driven inflammation.
Eur Heart J. 32 (20): 2573-84. -
Püntener, U. et al. (2012) Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.
J Neuroinflammation. 9: 146. -
Li, M. et al. (2014) The indoleamine 2,3-dioxygenase pathway controls complement-dependent enhancement of chemo-radiation therapy against murine glioblastoma.
J Immunother Cancer. 2: 21. -
Braach, N. et al. (2014) RAGE controls activation and anti-inflammatory signalling of protein C.
PLoS One. 9 (2): e89422. -
El Amki, M. et al. (2018) Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice.
Mol Neurobiol. 55 (12): 9156-68. -
Basic, M. et al. (2020) Lymph Node Stromal Cells From Different Draining Areas Distinctly Regulate the Development of Chronic Intestinal Inflammation.
Front Immunol. 11: 549473.
- Synonyms
- VCAM-1
- RRID
- AB_566429
- UniProt
- P29533
- Entrez Gene
- Vcam1
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0016021 integral to membrane
- GO:0007507 heart development
- GO:0031225 anchored to membrane
- GO:0044419 interspecies interaction between organisms
- GO:0060669 embryonic placenta morphogenesis
- GO:0060710 chorio-allantoic fusion
MCA2297GA
151779
163607
MCA2297
0413
151780
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