CD102 antibody | mIC2/4 (3C4)
Rat anti Mouse CD102 antibody, clone mIC2/4 (3C4) recognizes murine intercellular adhesion molecule-2 (ICAM-2), also known as CD102. Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation.
Rat anti Mouse CD102 antibody, clone mIC2/4 is reported to inhibit interactions between CD102 and LFA-1 (Xuet al. 1996).
Rat anti Mouse CD102 antibody, clone mIC2/4 is reported to inhibit interactions between CD102 and LFA-1 (Xuet al. 1996).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- COS cells transfected with murine ICAM-2.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35330
- Entrez Gene
- Icam2
- GO Terms
- GO:0001931 uropod
- GO:0005887 integral to plasma membrane
- GO:0016337 cell-cell adhesion
- Regulatory
- For research purposes only
Applications of CD102 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Functional Assays | |||
| Immunoprecipitation | |||
| Flow Cytometry | 1/50 | 1/200 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | F | 0.5 mg |
|
|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
Source Reference
-
Xu, H. et al. (1996) Characterization of murine intercellular adhesion molecule-2.
J Immunol. 156 (12): 4909-14.
References for CD102 antibody
-
Jones, D.T. et al. (2013) Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice.
Dis Model Mech. 6 (1): 115-24.
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