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ART2.2 antibody | Nika102

Rat anti Mouse ART2.2

Product Type
Monoclonal Antibody
Clone
Nika102
Isotype
IgG2a
Specificity
ART2.2

Product Code Applications Pack Size List Price Your Price Qty
MCA1975
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.25 mg loader
List Price Your Price
loader

Rat anti Mouse ART2.2 antibody, clone Nika102 recognizes the gene product of the mouse ADP-ribosyltransferase (ART) 2.2 gene, an ortholog of rat RT6. ART2.2 expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (Koch-Nolte et al. 1999).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
pME.CD8LF-ART2.2
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized wistar rats were fused with cells of the Sp2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

Source Reference

  1. Koch-Nolte, F. et al. (1999) A new monoclonal antibody detects a developmentally regulated mouse ecto-ADP-ribosyltransferase on T cells: subset distribution, inbred strain variation, and modulation upon T cell activation.
    J Immunol. 163 (11): 6014-22.

References for ART2.2 antibody

  1. Kahl, S. et al. (2000) Metalloprotease-mediated shedding of enzymatically active mouse ecto-ADP-ribosyltransferase ART2.2 upon T cell activation.
    J Immunol. 165:4463-9.
  2. Adriouch, S. et al. (2001) Rapid induction of naive T cell apoptosis by ecto-nicotinamide adenine dinucleotide: requirement for mono(ADP-ribosyl)transferase 2 and a downstream effector.
    J Immunol. 167:196-203
  3. Ablamunits, V. et al. (2001) Changing patterns of cell surface mono (ADP-ribosyl) transferase antigen ART2.2 on resting versus cytopathically-activated T cells in NOD/Lt mice.
    Diabetologia. 44 (7): 848-58.
  4. Adriouch, S. et al. (2007) NAD+ released during inflammation participates in T cell homeostasis by inducing ART2-mediated death of naive T cells in vivo.
    J Immunol. 179 (1): 186-94.
  5. Heiss K et al. (2008) High sensitivity of intestinal CD8+ T cells to nucleotides indicates P2X7 as a regulator for intestinal T cell responses.
    J Immunol. 181 (6): 3861-9.
  6. Menzel, S. et al. (2015) Nucleotide-Induced Membrane-Proximal Proteolysis Controls the Substrate Specificity of T Cell Ecto-ADP-Ribosyltransferase ARTC2.2.
    J Immunol. 195 (5): 2057-66.

RRID
AB_2258718
UniProt
O35975
Entrez Gene
Art2b
GO Terms
GO:0003956 NAD(P)+-protein-arginine ADP-ribosyltransferase activity
GO:0031225 anchored to membrane
GO:0018120 peptidyl-arginine ADP-ribosylation
GO:0019897 extrinsic to plasma membrane
GO:0051789 response to protein stimulus

MCA1975

148606 154547 167281

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