ABCA1 antibody | 5A1-1422
Rat anti Mouse ABCA1
- Product Type
- Monoclonal Antibody
- Clone
- 5A1-1422
- Isotype
- IgG2a
- Specificity
- ABCA1
Antibody Quality and Performance Guarantee
| Rat anti Mouse ABCA1 antibody, clone 5A1.1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangier disease, a genetic disorder of lipid metabolism, and the more common Familial HDL deficiency, peresenting with many of the same symproms. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- ABCA1 transfected HeLa cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/200 |
| Immunofluorescence | |
||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for ABCA1 antibody
-
Pradel, L.C. et al. (2009) ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs.
Eur J Immunol. 39 (8): 2270-80. -
Wang, Y. et al. (2019) Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.
Arterioscler Thromb Vasc Biol. 39 (5): 876-87.
Further Reading
-
Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.
Pflugers Arch. 453 (5): 569-79.
- RRID
- AB_1604583
- UniProt
- P41233
- Entrez Gene
- Abca1
- GO Terms
- GO:0002790 peptide secretion
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0005548 phospholipid transporter activity
- GO:0005794 Golgi apparatus
- GO:0006497 protein lipidation
- GO:0006911 phagocytosis, engulfment
- GO:0008203 cholesterol metabolic process
- GO:0008509 anion transmembrane transporter activity
- View More GO Terms
- GO:0016887 ATPase activity
- GO:0010875 positive regulation of cholesterol efflux
- GO:0017127 cholesterol transporter activity
- GO:0033344 cholesterol efflux
- GO:0033700 phospholipid efflux
- GO:0043691 reverse cholesterol transport
- GO:0045332 phospholipid translocation
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071300 cellular response to retinoic acid
MCA2681
150650
164083
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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