AATF antibody | 1B2D8
Rat anti Mouse AATF
- Product Type
- Monoclonal Antibody
- Clone
- 1B2D8
- Isotype
- IgG2a
- Specificity
- AATF
Antibody Quality and Performance Guarantee
| Rat anti Mouse AATF antibody, clone 1B2D8 recognizes apoptosis-antagonizing transcription factor, also known as Rb-binding protein che-1. AATF was first identified as an RNA pol II – interacting factor (Fanciulli et al. 2000). AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis (Kumar et al. 2019). DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012). Rat anti Mouse AATF antibody detects a band of 80 kDa. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide comprising amino acids 1-39 of mouse AATF (apoptosis-antagonizing transcription factor)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/100 | 1/500 |
| Western Blotting | |
2 ug/ml | 4 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- Q9JKX4
- Entrez Gene
- Aatf
- GO Terms
- GO:0042254 ribosome biogenesis
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0005730 nucleolus
- GO:0007346 regulation of mitotic cell cycle
- GO:0040016 embryonic cleavage
- GO:0042985 negative regulation of amyloid precursor protein biosynthetic process
MCA6375
160845
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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