AATF antibody | 1B2D8

Rat anti Mouse AATF

Product Type
Monoclonal Antibody
Clone
1B2D8
Isotype
IgG2a
Specificity
AATF

Product Code Applications Pack Size List Price Your Price Qty
MCA6375
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 0.1 mg loader
List Price Your Price
loader
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Rat anti Mouse AATF antibody, clone 1B2D8 recognizes apoptosis-antagonizing transcription factor, also known as Rb-binding protein che-1.

AATF was first identified as an RNA pol II – interacting factor (Fanciulli et al. 2000). AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis (Kumar et al. 2019). DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012).

Rat anti Mouse AATF antibody detects a band of 80 kDa.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide comprising amino acids 1-39 of mouse AATF (apoptosis-antagonizing transcription factor)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100 1/500
Western Blotting 2 ug/ml 4 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q9JKX4
Entrez Gene
Aatf
GO Terms
GO:0042254 ribosome biogenesis
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0007346 regulation of mitotic cell cycle
GO:0040016 embryonic cleavage
GO:0042985 negative regulation of amyloid precursor protein biosynthetic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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