Adalimumab antibody | AbD18655_hIgE

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Human anti Adalimumab

Anti-adalimumab antibody is a recombinant, inhibitory, high affinity anti-idiotypic antibody in fully human IgE format. It is recommended as a reference standard in ADA assays to measure levels of patient anti-drug antibodies with IgE isotype.

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Human anti Adalimumab, clone AbD18655_hIgE, is an ultra-high affinity, recombinant anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. This antibody is derived from the same parent clone as HCA204 and has been converted to an IgE isotype.

Repeated administration of biotherapeutic drugs can result in the induction of anti-drug antibodies (ADAs), which may affect efficacy of the drug and may cause adverse events including hypersensitivity reactions. Most ADAs have isotype IgG, but ADAs with isotype IgE can potentially elicit anaphylactic reactions. The FDA draft guidance for immunogenicity testing of therapeutic proteins indicates that where the risk of anaphylaxis is a concern, assays should be developed to investigate ADAs with IgE isotype.

Clone AbD18655_hIgE is a fully human antibody with isotype IgE, ideal for use as a reference standard in an ELISA to measure IgE ADAs in patient serum.

Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all anti-adalimumab antibodies.

Product Details

Product Form
Human IgE antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgE prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Adalimumab (Humira®)
The monovalent intrinsic affinity of this antibody was measured as KD=0.06 nM by real time, label-free molecular interaction analysis on immobilized adalimumab
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Humira is a registered trademark of Abbott Laboratories.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
For research purposes only

Applications of Adalimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
This product may be used in an indirect ELISA.
This antibody is fully human and can be used as a reference standard in an ADA assay.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human IgE:HRP MCA2116P E 0.5 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049B E IY 125 ml

Product Specific References

Further Reading

  1. Wu, Y., et al. (2013) An electrochemiluminescence (ECL)-based assay for the specific detection of anti-drug antibodies of the IgE isotype.
    J Pharm Biomed Anal. 86: 73-81.
  2. Puxeddu, I., et al. (2016) Hypersensitivity reactions during treatment with biological agents.
    Clin Exp Rheumatol.34: 129-32.

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