TNF Alpha antibody | D2E7
0.5 μg of Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) was performed on an analytical HPLC instrument. The larger peak corresponds to monomeric, fully assembled antibody (H2L2), the smaller peak contains oligomers. Column: MAbPac SEC-1.5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, 200mM arginine, 300mM NaCl, pH 6.8, flow rate: 200 μl/min; inj. volume: 3 μl; temp.: 25 °C; detection: 280 nm; sample: 1 mg/ml.
A microtiter plate was coated overnight with Human Anti-Adalimumab Antibody (clone AbD18654, HCA202) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab (therapeutic reference product) or Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Adalimumab Antibody (clone AbD18654_hIgG1, HCA203) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK001P).
Human anti TNF Alpha (Adalimumab Biosimilar)
Anti-TNF Alpha Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug adalimumab (Humira) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
- Product Type
- Monoclonal Antibody
- Clone
- D2E7
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6141 | E | 0.2 mg |
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Human Anti-TNF alpha antibody, clone D2E7 is a research grade biosimilar of the monoclonal antibody drug adalimumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic adalimumab antibody clone D2E7. This product is supplied in a research grade format, i.e. in PBS buffer with preservative; it is not manufactured or formulated in the same way as the therapeutic reference product.
Human Anti-TNF alpha antibody, clone D2E7 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271.
The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-adalimumab antibody products
Human Anti-TNF alpha antibody, clone D2E7 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271.
The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-adalimumab antibody products
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Human TNFα
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Adalimumab | HCA202 | E | 0.1 mg |
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| Human anti Adalimumab | HCA203 | E | 0.1 mg |
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| Human anti Adalimumab | HCA204 | E | 0.1 mg |
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| Human anti Adalimumab:HRP | HCA204P | E | 0.1 mg |
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| Human anti Adalimumab | HCA205 | E | 0.1 mg |
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| Human anti Adalimumab (Drug/Target Complex) | HCA206 | E | 0.1 mg |
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| Human anti Adalimumab (Drug/Target Complex) | HCA207 | E | 0.1 mg |
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| Human anti Adalimumab (Drug/Target Complex) | HCA231 | E | 0.1 mg |
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| Human anti Adalimumab (Drug/Target Complex) | HCA232 | E | 0.1 mg |
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| Human anti Adalimumab (Drug/Target Complex):HRP | HCA232P | E | 0.1 mg |
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| Human anti Adalimumab | HCA271 | E | 0.1 mg |
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody |
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