TNF Alpha antibody | D2E7
0.5 μg of Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) was performed on an analytical HPLC instrument. The larger peak corresponds to monomeric, fully assembled antibody (H2L2), the smaller peak contains oligomers. Column: MAbPac SEC-1.5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, 200mM arginine, 300mM NaCl, pH 6.8, flow rate: 200 μl/min; inj. volume: 3 μl; temp.: 25 °C; detection: 280 nm; sample: 1 mg/ml.
A microtiter plate was coated overnight with Human Anti-Adalimumab Antibody (clone AbD18654, HCA202) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab (therapeutic reference product) or Human Anti-TNFα Antibody, MCA6141 (adalimumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Adalimumab Antibody (clone AbD18654_hIgG1, HCA203) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK001P).
Human anti TNF Alpha (Adalimumab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- D2E7
- Isotype
- IgG1
- Specificity
- TNF Alpha
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| Human Anti-TNF alpha antibody, clone D2E7 is a research grade biosimilar of the monoclonal antibody drug adalimumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic adalimumab antibody clone D2E7. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. Human Anti-TNF alpha antibody, clone D2E7 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271. The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-adalimumab antibody products |
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Human TNFα
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
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