IL-10 antibody | JES3-12G8

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Rat anti Human Interleukin-10:Preservative Free

Rat anti Human Interleukin-10

Product Type
Monoclonal Antibody
Clone
JES3-12G8
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2250XZ E F* FN P* WB 1 mg
MCA2250 E F* P* WB 0.5 mg
Rat anti Human Interleukin-10 antibody, clone JES3-12G8 recognizes human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes.

Rat anti Human Interleukin-10 antibody, clone JES3-12G8 is reported to neutralize the effects of IL-10 (Abrams et al. 1992).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human IL-10 expressed by COS cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P22301 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
Functional Assays
Immunohistology - Paraffin 2 1/100
Western Blotting 1/100 1/250
ELISA
Flow Cytometry 1
Immunohistology - Paraffin 2 1/50 1/100
Western Blotting 1/50 1/250
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil
Histology Positive Control Tissue
Human Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for IL-10 antibody

  1. Brodeur, N.D. and Spencer, J.V. (2010) Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity.
    Virus Res. 153: 265-8.
  2. Burdin, N. et al. (1993) Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10.
    J Exp Med. 177 (2): 295-304.
  3. O'farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
    EMBO J. 17 (4): 1006-18.
  4. Abrams, J. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol. Rev. 127:5-24.
  5. Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
    J Immunol. 167: 3107-13.
  6. Dybiec, M. et al. (2007) Photoluminescence spectroscopy of bioconjugated CdSe/ZnS quantum dots
    Appl. Phys. Lett. 90, 263112.
  7. Ramgir, N.S. et al. (2007) Voltammetric Detection of Cancer Biomarkers Exemplified by Interleukin-10 and Osteopontin with Silica Nanowires
    J. Phys. Chem. C, 111:13981–7.
  8. Zajac, A. <>et al. (2007) Protein microarrays and quantum dot probes for early cancer detection.
    Colloids Surf B Biointerfaces. 58: 309-14.

Further Reading

  1. Gomes, J. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
    Infect. Immun. 71:1185-1193.
  2. Yssel, H. et al. (1992) IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.
    J Immunol. 149 (7): 2378-84.