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IL-10 antibody | JES3-12G8

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Anti Human Interleukin-10 Antibody, clone JES3-12G8 thumbnail image 1 Anti Human Interleukin-10 Antibody, clone JES3-12G8 thumbnail image 2
Anti Human Interleukin-10 Antibody, clone JES3-12G8 gallery image 1
Western blot analysis of human IL-10 recombinant protein probed with Rat anti Human Interleukin-10 (MCA2250) followed by F(ab')2 Rabbit anti Rat IgG:HRP (STAR21B)
Anti Human Interleukin-10 Antibody, clone JES3-12G8 gallery image 2
Published customer image:
Rat anti Human Interleukin-10 antibody, clone JES3-12G8 (MCA2250) used for the demonstration of IL-10 producing cells in sections of anthrocosis associated lymph node tissues by immunohistochemistry on formalin fixed, paraffin embedded material.
Image caption:
Tumor-promoting phenotype of the myeloid clusters.
IHC staining demonstrating the expression of CD163 and IL-10 by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P<,0.001. (Scale bar, 200 μm for 100× and 400×; 25 μm for 1600×.

From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, Bhanji S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Human Interleukin-10

Product Type
Monoclonal Antibody
Clone
JES3-12G8
Isotype
IgG2a
Specificity
IL-10

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Product Code Applications Pack Size List Price Your Price Qty
MCA2250
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * P * WB 0.5 mg loader
List Price Your Price
loader

Rat anti Human Interleukin-10 antibody, clone JES3-12G8 recognizes human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes.

Rat anti Human Interleukin-10 antibody, clone JES3-12G8 is reported to neutralize the effects of IL-10 (Abrams et al. 1992).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human IL-10 expressed by COS cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
Immunohistology - Paraffin 2 1/50 1/100
Western Blotting 1/50 1/250
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for IL-10 antibody

  1. Burdin, N. et al. (1993) Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10.
    J Exp Med. 177 (2): 295-304.
  2. Yssel, H. et al. (1992) IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.
    J Immunol. 149 (7): 2378-84.
  3. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  4. O'Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
    EMBO J. 17 (4): 1006-18.
  5. Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
    J Immunol. 167: 3107-13.
  6. Zajac, A. <>et al. (2007) Protein microarrays and quantum dot probes for early cancer detection.
    Colloids Surf B Biointerfaces. 58: 309-14.
  7. Dybiec, M. et al. (2007) Photoluminescence spectroscopy of bioconjugated CdSe∕ZnS quantum dots
    Applied Physics Letters. 90 (26): 263112.
  8. Ramgir, N.S. et al. (2007) Voltammetric Detection of Cancer Biomarkers Exemplified by Interleukin-10 and Osteopontin with Silica Nanowires
    The Journal of Physical Chemistry C. 111 (37): 13981-7.
    9. View The Latest Product References
  9. van der Vlugt, L.E.P.M. et al. (2014) CD24(hi)CD27(+) B cells from patients with allergic asthma have impaired regulatory activity in response to lipopolysaccharide.
    Clin Exp Allergy. 44 (4): 517-28.
  10. Prosniak, M. et al. (2013) Glioma grade is associated with the accumulation and activity of cells bearing M2 monocyte markers.
    Clin Cancer Res. 19 (14): 3776-86.
  11. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
    PLoS One. 8 (5): e65121.
  12. van der Vlugt, E.P.M. et al. (2014) Toll-like receptor ligation for the induction of regulatory B cells.
    Methods Mol Biol. 1190: 127-41.
  13. Ramos, R.N. et al. (2020) CD163+ tumor-associated macrophage accumulation in breast cancer patients reflects both local differentiation signals and systemic skewing of monocytes.
    Clin Transl Immunology. 9 (2): e1108.

Further Reading

  1. Gomes, J.A. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
    Infect Immun. 71 (3): 1185-93.

RRID
AB_2125383
UniProt
P22301
Entrez Gene
IL10
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus

MCA2250

154573 1803 1806

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