Evolocumab antibody | AbD40406ia

Human anti Evolocumab

Product Type
Monoclonal Antibody
Clone
AbD40406ia
Isotype
IgG1
Specificity
Evolocumab

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New HCA377
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Evolocumab Antibody, clone AbD40406ia, is a paratope specific, inhibitory antibody that specifically recognizes the monoclonal antibody drug evolocumab. It does not recognize the drug target, human proprotein convertase subtilisin/kexin type 9 (PCSK9), nor evolocumab in complex with PCSK9.

Clone AbD40406ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 1 nM and is one of three antibodies offered for ADA assays. Antibody HCA376 and HCA378 both have an affinity of 5 nM.

Evolocumab (Repatha) is a fully human IgG2 lambda antibody developed using transgenic mouse technology. It has been approved for treating selected patients with high cholesterol, and for the prevention of heart attack and stroke. The drug prevents the PCSK9 protein binding to the low density lipoprotein receptor (LDLR), thereby increasing the number of LDLRs available and lowering LDL levels.

View a summary of all evolocumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Evolocumab
Affinity
The monovalent intrinsic affinity of AbD40406ia was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized evolocumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Repatha is a trademark of Amgen.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD40406ia can be used as a surrogate positive control or calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
List Price Your Price
loader
Description Mouse anti Human IgG (Fc) CH2 Domain:HRP
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Evolocumab HCA378 E 0.1 mg loader
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loader
Description Human anti Evolocumab
Human anti Evolocumab HCA376 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab
Human anti Evolocumab TZA007 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab
Human anti Evolocumab TZA008 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab

Synonyms
Repatha
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Evolocumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"