Cemiplimab antibody | AbD41947ad
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, Human Anti-Cemiplimab Antibody, clone AbD41947ad (TZA006) at a concentration of 2 μg/ml was added. Detection was performed using HRP conjugated anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with cemiplimab, human IgG4 kappa (S228P) isotype control or pembrolizumab at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Cemiplimab Antibody, clone AbD41947ad (TZA006) titrated to the given concentrations in PBST, followed by Mouse Anti- Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human PD-1 at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of cemiplimab (0.15 µg/ml) plus increasing concentrations of Human Anti-Cemiplimab Antibody, clone AbD41947ad (TZA006) was added. Free cemiplimab, still capable of binding to the human PD-1 coated plate, was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Cemiplimab Antibody, clone AbD41947ad (TZA006) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of cemiplimab. Detection was performed using HRP conjugated Human Anti-Cemiplimab Antibody, clone AbD41947ia (HCA368) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
Human anti Cemiplimab
- Product Type
- Monoclonal Antibody
- Clone
- AbD41947ad
- Isotype
- HuCAL Fab monovalent
- Specificity
- Cemiplimab
Antibody Quality and Performance Guarantee
| Human Anti-Cemiplimab Antibody, clone AbD41947ad is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody cemiplimab. It does not recognize free human programmed death receptor-1 (PD-1) nor cemiplimab in complex with PD-1. The antibody can be used to measure free cemiplimab in patient samples. The antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. A pair of anti-cemiplimab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody can be used as a capture reagent, paired with Anti-Cemiplimab Antibody, clone AbD41947ia (HCA368) in full IgG1 format as the detection reagent. View a summary of SpyCatcher products Cemiplimab (Libtayo) is a fully human IgG4/kappa antibody. It has been approved for the treatment of patients with metastatic cutaneous squamous cell carcinoma (CSCC) and for the first-line treatment of patients with advanced non-small cell lung cancer (NSCLC) whose tumours have high PD-L1 expression. Cemiplimab acts as a checkpoint inhibitor by binding to the PD-1 receptor and blocking its interaction with PD-L1 and PD-L2 ligands. View a summary of all anti-cemiplimab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library. Expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% ProClin 950
- Immunogen
- Cemiplimab
- Affinity
- The monovalent intrinsic affinity of AbD41947ad was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized cemiplimab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
His-tag is a registered trademark of EMD Biosciences.
Libtayo is a trademark of Regeneron Pharmaceuticals, Inc.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD41947ad can be used as the capture antibody for cemiplimab in a bridging ELISA together with HCA368 as the detection reagent.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
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| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Cemiplimab | HCA367 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
| Human anti Cemiplimab | HCA368 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
| Human anti Cemiplimab | HCA369 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
- Synonyms
- Libtayo
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
TZA006
160412
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