Cemiplimab antibody | AbD41951ia
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% non-fat dry milk, detection was performed using HRP conjugated Human Anti-Cemiplimab Antibody, clone AbD41951ia (HCA369) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with cemiplimab, human IgG4 kappa (S228P) isotype control or pembrolizumab at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Cemiplimab Antibody, clone AbD41951ia (HCA369) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with human PD-1 at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of cemiplimab (0.15 µg/ml) plus increasing concentrations of Human Anti-Cemiplimab Antibody, clone AbD41951ia (HCA369) in the monovalent Fab format was added. Free cemiplimab, still capable of binding to the human PD-1 coated plate, was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with cemiplimab at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Cemiplimab Antibody, clone AbD41947ia (HCA368, black), clone AbD41949ia (HCA367, blue) or clone AbD41951ia (HCA369, red). Detection was performed using HRP conjugated cemiplimab followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
Human anti Cemiplimab
- Product Type
- Monoclonal Antibody
- Clone
- AbD41951ia
- Isotype
- IgG1
- Specificity
- Cemiplimab
Antibody Quality and Performance Guarantee
| Human Anti-Cemiplimab Antibody, clone AbD41951ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody cemiplimab. It does not recognize free human programmed death receptor-1 (PD-1) nor cemiplimab in complex with PD-1. The antibody can be used to measure free cemiplimab in patient samples. Clone AbD41951ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 9 nM, and is one of three antibodies with different affinities offered for ADA assays. Cemiplimab (Libtayo) is a fully human IgG4/kappa antibody. It has been approved for the treatment of patients with metastatic cutaneous squamous cell carcinoma (CSCC) and for the first-line treatment of patients with advanced non-small cell lung cancer (NSCLC) whose tumours have high PD-L1 expression. Cemiplimab acts as a checkpoint inhibitor by binding to the PD-1 receptor and blocking its interaction with PD-L1 and PD-L2 ligands. View a summary of all anti-cemiplimab antibodies |
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- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Cemiplimab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Cemiplimab antibody, clone AbD41951ia is KD = 9 nM as measured by real time, label free molecular interaction analysis on immobilized Cemiplimab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Libtayo is a trademark of Regeneron Pharmaceuticals, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Cemiplimab antibody, clone AbD41951ia can be used as a calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Cemiplimab | HCA367 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
| Human anti Cemiplimab | HCA368 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Human anti Cemiplimab | TZA006 | E | 0.1 mg |
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| Description | Human anti Cemiplimab | ||||||
- Synonyms
- Libtayo
HCA369
160411
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CEMIPLIMAB specificity
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