Cemiplimab antibody | AbD41947ia

Human anti Cemiplimab

Product Type
Monoclonal Antibody
Clone
AbD41947ia
Isotype
IgG1
Specificity
Cemiplimab

Product Code Applications Pack Size List Price Your Price Qty
HCA368
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E 0.1 mg loader
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Human Anti-Cemiplimab Antibody, clone AbD41947ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody cemiplimab. It does not recognize free human programmed death receptor-1 (PD-1) nor cemiplimab in complex with PD-1. The antibody can be used to measure free cemiplimab in patient samples.

Clone AbD41947ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 2 nM, and is one of three antibodies with different affinities offered for ADA assays.

A pair of anti-cemiplimab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody can be used as a detection reagent, paired with Anti-Cemiplimab Antibody, clone AbD41947ad (TZA006) in monovalent Fab format as the capture antibody.

Cemiplimab (Libtayo) is a fully human IgG4/kappa antibody. It has been approved for the treatment of patients with metastatic cutaneous squamous cell carcinoma (CSCC) and for the first-line treatment of patients with advanced non-small cell lung cancer (NSCLC) whose tumours have high PD-L1 expression. Cemiplimab acts as a checkpoint inhibitor by binding to the PD-1 receptor and blocking its interaction with PD-L1 and PD-L2 ligands.

View a summary of all anti-cemiplimab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Cemiplimab
Affinity
The intrinsic affinity of the monovalent form of Human anti Cemiplimab antibody, clone AbD41947ia is KD = 2 nM as measured by real time, label free molecular interaction analysis on immobilized Cemiplimab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Libtayo is a trademark of Regeneron Pharmaceuticals, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Cemiplimab antibody, clone AbD41947ia can be used as detection antibody for Cemiplimab in a bridging ELISA together with TZA006 as the capture reagent. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.
Protocol: PK bridging ELISA.
Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Cemiplimab HCA369 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Cemiplimab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Cemiplimab TZA006 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Cemiplimab
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Cemiplimab HCA367 E 0.1 mg loader
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loader
Description Human anti Cemiplimab

Synonyms
Libtayo
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"