IFN Gamma Antibody

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IFN Gamma Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Rabbit anti Human interferon-gamma (AHP775) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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IFN Gamma Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Rabbit anti Human interferon-gamma (AHP775) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • IFN Gamma Antibody thumbnail image 1
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  • Rabbit anti Human Interferon Gamma
  • Rabbit anti Human Interferon Gamma:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP775E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP775
    AHP775BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP775B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Interferon gamma antibody recognizes human IFN-gamma (Interferon-gamma), a 143 amino acid regulatory cytokine and member of the Type II interferon family. IFN-gamma is involved in the regulation of the immune and inflammatory responses and is secreted primarily by mitogen and antigen activated T lymphocytes and natural killer cells (NK).

      IFN-gamma is a multifunctional cytokine which signals through the IFN-gamma receptor (IFNGR), found on most immune cells. Properties of IFN-gamma include recruitment of leucocytes to sites of infection, inducement of MHC class I and II antigens, stimulation of anti-microbial and anti-tumour responses of macrophages, Nk cells and neutrophils, and has also been shown to potentiate the antiproliferative effects of IFN-alpha and IFN-beta.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human IFNγ were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution
      IgG concentration 0.1 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      negative regulation of metanephric nephron tubule epithelial cell differentiation
      cell cycle arrest
      positive regulation of interleukin-23 production
      tyrosine phosphorylation of Stat1 protein
      positive regulation of interleukin-12 production
      positive regulation of mesenchymal cell proliferation
      positive regulation of osteoclast differentiation
      positive regulation of tyrosine phosphorylation of Stat1 protein
      positive regulation of membrane protein ectodomain proteolysis
      positive regulation of calcidiol 1-monooxygenase activity
      interferon-gamma receptor binding
      negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      interferon-gamma-mediated signaling pathway
      protein import into nucleus, translocation
      positive regulation of fructose 1,6-bisphosphate metabolic process
      positive regulation of peptidyl-serine phosphorylation of STAT protein
      positive regulation of nitric oxide biosynthetic process
      positive regulation of vitamin D biosynthetic process
      positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
      regulation of insulin secretion
      positive regulation of smooth muscle cell apoptosis
      immune response
      cytokine activity
      positive regulation of killing of cells of other organism
      extracellular space
      negative regulation of smooth muscle cell proliferation
      regulation of interferon-gamma-mediated signaling pathway
      response to virus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.040.06ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human IFNγ antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IFNγ antibody (AHP775B) as the detection reagent and recombinant human IFNγ (PHP050) as the standard.
    • ELISA
      This biotinylated human IFNγ antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IFNγ antibody (AHP775) as the capture reagent and recombinant human IFNγ (PHP050) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IFNγ (PHP050) as the positive control.
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IFNγ (PHP050) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Applications Sizes available
    IFN Gamma Antibody : Biotin E, WB 50 µg
    IFN Gamma Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human Interferon Gamma:BiotinAHP775B50 µgE, WB
          AHP775B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interferon GammaAHP7750.1 mgE, FN, P *, WB
          AHP775

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interferon GammaPHP0500.1 mgE, FN, WB
            PHP050
            Recombinant Human Interferon GammaPHP050A20 µgE, FN, WB
            PHP050A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interferon GammaPHP0500.1 mgE, FN, WB
            PHP050
            Recombinant Human Interferon GammaPHP050A20 µgE, FN, WB
            PHP050A

            Histology Controls

              Further Reading

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