IFN Gamma

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  • Recombinant Human Interferon Gamma
  • Recombinant Human Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP050E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    PHP050
    PHP050AE, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP050A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • IFN-gamma is a lymphoid factor with anti viral activity and macrophage and NK cell stimulating properties.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Recombinant protein produced in E. coli - lyophilised.
      Recombinant protein produced in E. coli - lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 20 µgReconstitute with 20ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 0.1 mgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 20 µgReconstitute with 20ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
      None present
    • Activity
      > 2 x 107 units/mg
    • Purity
      >98% by SDS PAGE and HPLC
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgTotal protein concentration 1.0mg/ml after reconstitution.
      Pack Size: 20 µg1.0 mg/ml after reconstitution
      Pack Size: 0.1 mgTotal protein concentration 1.0mg/ml after reconstitution.
      Pack Size: 20 µg1.0 mg/ml after reconstitution
    • Protein Molecular Weight
      16.7kD (143 Amino Acid Sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      Pack Size: 0.1 mgPhosphate buffered saline
      Pack Size: 20 µgSodium Phosphate
      Pack Size: 0.1 mgPhosphate buffered saline
      Pack Size: 20 µgSodium Phosphate
    • Storage
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 20 µgPrior to reconstitution store at +4oC.

      Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 20 µgPrior to reconstitution store at +4oC.

      Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution
      3 months from date of reconstitution
    • GO Terms
      negative regulation of metanephric nephron tubule epithelial cell differentiation
      interferon-gamma-mediated signaling pathway
      positive regulation of osteoclast differentiation
      positive regulation of peptidyl-serine phosphorylation of STAT protein
      positive regulation of smooth muscle cell apoptosis
      cell cycle arrest
      negative regulation of smooth muscle cell proliferation
      response to virus
      tyrosine phosphorylation of Stat1 protein
      positive regulation of nitric oxide biosynthetic process
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      positive regulation of killing of cells of other organism
      positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
      positive regulation of mesenchymal cell proliferation
      positive regulation of vitamin D biosynthetic process
      extracellular space
      negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
      positive regulation of fructose 1,6-bisphosphate metabolic process
      regulation of interferon-gamma-mediated signaling pathway
      positive regulation of membrane protein ectodomain proteolysis
      regulation of insulin secretion
      interferon-gamma receptor binding
      cytokine activity
      positive regulation of calcidiol 1-monooxygenase activity
      protein import into nucleus, translocation
      positive regulation of interleukin-23 production
      immune response
      positive regulation of tyrosine phosphorylation of Stat1 protein
      positive regulation of interleukin-12 production
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110ng/ml
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      PHP050A may be used in ELISA applications with either AHP775 or AHP775B.
    • ELISA
      PHP050 may be used in ELISA applications with either AHP775 or AHP775B.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      PHP050A may be used under reducing or non-reducing western blotting conditions with either AHP775 or AHP775B.
    • Western Blotting
      PHP050 may be used under reducing or non-reducing western blotting conditions with either AHP775 or AHP775B.
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Rec. Protein E, FN, WB 20 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interferon Gamma:BiotinAHP775B50 µgE, WB
          AHP775B
          Rabbit anti Human Interferon GammaAHP7750.1 mgE, FN, P *, WB
          AHP775
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interferon Gamma:BiotinAHP775B50 µgE, WB
          AHP775B
          Rabbit anti Human Interferon GammaAHP7750.1 mgE, FN, P *, WB
          AHP775

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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