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Caspase-3 antibody

Rabbit anti Caspase-3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Format
Purified
Specificity
Caspase-3

Product Code Applications Pack Size List Price Your Price Qty
AHP2717
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P WB 50 µl loader
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Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3, as the name implies, is a member of the cysteine-aspartic acid protease (caspase) protein family.
Caspase-3 is an effector caspase that is activated by both the intrinsic and extrinsic apoptosis pathways. Caspase-3 is cleaved from an inactive 32 kDa zymogen/pro-enzyme into p17 and p12 subunits by the activated initiator caspases 9 and 8 in apoptotic cells. These subunits dimerize to form the active caspase 3 protein that cleaves and activates caspase-6 and 2. Through the activation/cleavage of downstream caspases, caspase-3 plays an important role in mediating apoptosis signaling.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti caspase-3 antibody detects a band of approximately 32 kDa in Jurkat cell lysate under reducing conditions
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to caspase-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% ProClin 300
50% Glycerol
Immunogen
Recombinant human caspase-3
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P42574
Entrez Gene
CASP3
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006508 proteolysis
GO:0008624 induction of apoptosis by extracellular signals
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
GO:0034612 response to tumor necrosis factor
GO:0043066 negative regulation of apoptosis
GO:0048011 nerve growth factor receptor signaling pathway

AHP2717

141649 154703 158224 163879

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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