DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.
Newborn rat primary cardiac myocytes stained with mouse anti-desmin, detected with goat anti-mouse IgG:DyLight® 488 (STAR74D488); rat anti-alpha tubulin detected with goat anti-rat IgG:DyLight® 649 (STAR71D649); and rabbit anti-MyBP-C detected with sheep anti-rabbit IgG: DyLight® 549.
DyLight® dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry, and may also be used in ELISA, Western blotting, high throughput screening and other array platforms.
Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.
We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.
Dye | Excitation max (nm) | Emission max (nm) | Fluorescence color |
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DyLight® 405 | 400 | 405 | |
DyLight® 488 | 493 | 518 | |
DyLight® 550 | 562 | 576 | |
DyLight® 650 | 652 | 672 | |
DyLight® 680 | 692 | 712 | Near IR |
DyLight® 800 | 777 | 794 | Near IR |
Description | Specificity | Target | Format | Host | Isotype | Clone | Applications | Citations | Product Type | Code | Validation Types |
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Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.