Alexa Fluor® 647 dyes, part of our Alexa Fluor fluorescent conjugates range, give you a superior far-red fluorophore that is commonly used in flow cytometry and immunofluorescence / confocal microscopy applications.
Fig. 1. Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 antibody, FITC (MCA497F) at a 1/5 dilution and CD11b antibody (MCA74A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock (BUF041B).
Sometimes referred to as AF647, Alexa Fluor 647 dyes are:
|Maximal absorbance (nm)
|Maximal emission (nm)
|Alexa Fluor 647
Bio-Rad offers primary antibodies conjugated to Alexa Fluor 647 across human, hamster, mouse, and rat catalog ranges.
Alexa Fluor 647 dye molecules have the ability to bind to proteins in high molar ratios without considerable self-quenching. This results in the formation of more radiant conjugates and improved detection sensitivity. Due to these properties, it is an outstanding option for the highly sensitive detection of biological structures that are present in low quantities.
Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.