Alexa Fluor 488–Conjugated Antibodies
Overview
Alexa Fluor 488 dyes, part of our Alexa Fluor fluorescent conjugates range, give you outstanding results in flow cytometry and immunofluorescence/confocal microscopy.
Alexa Fluor 488 dyes, sometimes referred to as AF488, are:
- The finest green fluorophore available
- Excited by an argon-ion laser
- Usually detected in the same fluorescence channel as FITC
- Alternative for fluorescein conjugates (FITC, FAM) and Cy2
- Brighter and more photostable than fluorescein
Fig.1. Immunofluorescence image of mouse small intestine stained with Rat Anti-Mouse F4/80 (MCA497PE), red and Rat Anti-Mouse CD45 (MCA1031A488), green; nuclei are stained with DAPI, blue.
The image highlights macrophages within the lamina propria.
Technical Features of Alexa Fluor 488 Dye
| Fluorochrome | Maximal absorbance (nm) | Maximal emission (nm) | Flow cytometry | Confocal microscopy | Color |
|---|---|---|---|---|---|
| Alexa Fluor 488 | 495 | 519 | Yes | Yes | Green |
Antibodies Conjugated to Alexa Fluor 488
Bio-Rad offers primary antibodies conjugated to Alexa Fluor 488 across human, mouse, and rat catalog ranges.
Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance, please do not hesitate to contact us.
AF488 Conjugated Antibodies
| Description | Target | Format | Clone | Applications | Citations | Code |
|---|
Fluorophore Dyes
Other dye formats available in our flow cytometry range include:
More details on emission and excitation values can be found on our Fluorophores page.
Flow Cytometry Basics Guide
Practical advice to get you started in flow cytometry
Keep up to date with recent advances by downloading our popular Flow Cytometry Basics Guide, which not only provides practical advice and best practice examples when planning multicolor experiments, but also a basic overview of all the important flow cytometry principles.
This comprehensive guide is split into nine easy-to-read chapters covering 54 pages, making this ideal for beginners wanting to gain confidence when planning experiments, and can also be used as a teaching aid.
