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Alexa Fluor® Antibody Conjugates
Fig 3.Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MCA497PE), red and Rat anti Mouse CD45 (MCA1031A488), green; nuclei are stained with DAPI, blue.
The image highlights macrophages within the lamina propria.
Alexa Fluor® conjugated antibodies produce outstanding results in flow cytometry, immunofluorescence and immunohistochemistry experiments.
Some of the main features of Alexa Fluor® fluorophores are:
- Brightness - brighter and more fluorescent than conventional dyes
- Photostability - more photostable than conventional fluorophores such as FITC and PE
- pH stability - insensitive to pH changes over a broad range
- Narrow emission spectra - ideal for multicolor and multi-plexing experiments
Technical Features of Alexa Fluor® Conjugates
| Fluorophores | Max excitation (nm) | Max emission (nm) | Color |
|---|---|---|---|
| Alexa Fluor® 405 | 401 | 421 | |
| Alexa Fluor® 488 | 495 | 519 | |
| Alexa Fluor® 647 | 650 | 665 | |
| Alexa Fluor® 700 | 702 | 723 | |
| PE-Alexa Fluor® 647 | 496, 546 | 667 | |
| PE-Alexa Fluor® 750 | 496, 546 | 779 |
Alexa Fluor® Conjugate Products
| Description | Target | Format | Clone | Applications | Citations | Code |
|---|
Alexa Fluor ® is a registered trademark of Molecular Probes Inc. OR, USA
DyLight ® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.