Matched Antibody Pairs for Bovine Cytokine ELISA
Biomarker Expression Patterns Posters
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Cytokines act as intracellular communication signals in a variety of processes including hematopoiesis, inflammation, immunity, and embryonic development. Measured in cattle sera, plasma and cell culture supernatants they monitor response to vaccination or diseases, such as tuberculosis and mastitis.
We have tested our anti-cytokine antibodies for use in sandwich ELISA applications, and recommend antibodies that work well as a matched pair. We also offer recombinant bovine cytokines as controls and for generation of a standard curve in the ELISA protocol. See the table below for our matched cytokine pairs. The interferon gamma antibodies are also available as a convenient ELISA kit.
Bovine Cytokine Antibody Pairs for ELISA
| Specificity & Format | Format | Clone |
|---|---|---|
| IFN alpha A (capture) | Purified | Polyclonal |
| IFN alpha A (detection) | Biotin | Polyclonal |
| IFN beta (capture) | Purified | Polyclonal |
| IFN beta (detection) | Biotin | Polyclonal |
| IFN gamma (capture) | Purified | CC330 |
| IFN gamma (detection) | Biotin | CC302 |
| IL-1 alpha(capture) | Purified | Polyclonal |
| IL-1 alpha (detection) | Biotin | Polyclonal |
| IL-1F5 (capture) | Purified | Polyclonal |
| IL-1F5 (detection) | Biotin | Polyclonal |
| IL-1RA (capture) | Purified | Polyclonal |
| IL-1RA (detection) | Biotin | Polyclonal |
| IL-4 (capture) | Purified | CC313 |
| IL-4 (detection) | Biotin | CC314 |
| IL-6 (capture) | Purified | Polyclonal |
| IL-6 (detection) | Biotin | Polyclonal |
| IL-10 (capture) | Purified | CC318 |
| IL-10 (detection) | Biotin | CC320 |
| IL-13 (capture) | Purified | Polyclonal |
| IL-13 (detection) | Biotin | Polyclonal |
| IL-15 (capture) | Purified | Polyclonal |
| IL-15 (detection) | Biotin | Polyclonal |
| IL-17A (capture) | Purified | Polyclonal |
| IL-17A (detection) | Biotin | Polyclonal |
| IL-21 (capture) | Purified | Polyclonal |
| IL-21 (detection) | Biotin | Polyclonal |
| TNF-alpha (capture) | Purified | CC327 |
| TNF-alpha (detection) | Biotin | CC328 |
| TNF alpha (capture) | Purified | Polyclonal |
| TNF alpha (detection | Biotin | Polyclonal |
Additional Bovine Cytokine Antibodies
| Specificity & Format | Clone | Applications |
|---|---|---|
| GM-CSF Purified | CC305 | E, WB |
| IFN gamma Purified | 7B6 | F, P, WB |
| IL-4 Purified | CC303 | E, F |
| IL-4 FITC | CC303 | F |
| IL-4 R-PE | CC303 | F |
| *IL-1 beta Purified | 1D4 | C, E, F, WB |
| IL-6 Purified | CC310 | E |
| *IL-8 Purified | 8M6 | E, F, WB |
| IL-12 Purified | CC301 | E, ES, F, FN, WB |
| IL-12 Azide Free | CC326 | E, F, FN |
| IL-12 Biotin | CC326 | E, F |
| *TGF-beta | 1D11 | E, FN, P |
Key to Applications: C immunohistology – frozen, E ELISA, ES ELISpot, F flow cytometry, FN functional assays, P immunohistology – paraffin, WB Western blotting
* Species cross reactivity demonstrated
