CD21 Antibody | CC51

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CD21 Antibody | CC51 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21 (MCA5953GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD21 Antibody | CC51 gallery image 2

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21:FITC (MCA5953F)

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CD21 Antibody | CC51 gallery image 3

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21:RPE (MCA5953PE)

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  • CD21 Antibody | CC51 thumbnail image 1
  • CD21 Antibody | CC51 thumbnail image 2
  • CD21 Antibody | CC51 thumbnail image 3
  • Mouse anti Bovine CD21:FITC
  • Mouse anti Bovine CD21
  • Mouse anti Bovine CD21:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    CC51
  • Isotype
    IgG2b
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5953FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5953F
    MCA5953GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA5953GA
    MCA5953PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5953PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012).

      Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      African Buffaloyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Immunogen
      Bovine (Friesian cattle) mesenteric lymph node cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch
      12 months from date of reconstitution
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD21 Antibody Formats

    Formats Clone Applications Sizes available
    CD21 Antibody : FITC CC51 F 0.1 mg
    CD21 Antibody : RPE CC51 F 100 Tests
    CD21 Antibody : Purified CC51 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat anti Human CD3:Pacific Blue®MCA1477PB100 Tests/1mlF *
          MCA1477PB
          Mouse anti Human CD14:Pacific Blue®MCA1568PB100 Tests/1mlF
          MCA1568PB
          Mouse anti Pig CD25MCA1736GA0.1 mgC, F
          MCA1736GA
          Mouse anti Pig SLA Class II DRMCA2314GA0.1 mgC, F, IP
          MCA2314GA
          Mouse anti Pig CD3MCA5951GA0.1 mgC, F, IP, WB
          MCA5951GA
          Mouse anti Pig wCD8 Alpha:RPEMCA1223PE100 TestsF
          MCA1223PE
          Mouse anti Human CD14:RPEMCA1568PE100 TestsF
          MCA1568PE
          Mouse anti Bovine CD4:RPEMCA1653PE100 TestsF
          MCA1653PE
          Mouse anti Pig CD4 Alpha:RPEMCA1749PE100 TestsF
          MCA1749PE
          Mouse anti Bovine CD25:RPEMCA2430PE100 TestsF
          MCA2430PE
          Mouse anti Bovine MHC Class II DR:RPEMCA5656PE100 TestsF
          MCA5656PE
          Mouse anti Pig CD3:RPEMCA5951PE100 TestsF
          MCA5951PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD14:FITCMCA1218F0.1 mgF
          MCA1218F
          Mouse anti Pig SLA Class II DR:FITCMCA2314F0.1 mgF
          MCA2314F
          Rat anti Human CD3:Pacific Blue®MCA1477PB100 Tests/1mlF *
          MCA1477PB
          Mouse anti Pig CD4 AlphaMCA1749GA0.1 mgC *, F
          MCA1749GA
          Mouse anti Pig wCD8 Alpha:RPEMCA1223PE100 TestsF
          MCA1223PE
          Mouse anti Bovine CD25:RPEMCA2430PE100 TestsF
          MCA2430PE
          Mouse anti Bovine MHC Class II DR:RPEMCA5656PE100 TestsF
          MCA5656PE
          Mouse anti Pig CD3:RPEMCA5951PE100 TestsF
          MCA5951PE

          Recommended Positive Controls

            Histology Controls

              References

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