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Bovine Dendritic Cell Growth Kit

Bovine Dendritic Cell Growth Kit gallery image 1

Bovine CD14 enriched cells visualised by light microscopy. A - 2 day cultured unstimulated cells; B - 2 day cultured cells in bovine dendritic cell growth kit (PBP015KZZ) at 1:20 dilution

  • Bovine Dendritic Cell Growth Kit thumbnail image 1
  • Bovine Dendritic Cell Growth Kit
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  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP015KZZFNdatasheet pdfdatasheet pdf5 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • PBP015KZZ is contains a cocktail of biologically active interleukin-4 (IL-4) and granulocyte/macrophage-colony stimulating factor (GM-CSF) that have been premixed at optimal concentrations to induce dendritic cell development from peripheral blood-derived bovine (cattle) monocytes.
    • Intended Use
    • Target Species
    • Product Form
      Mixed recombinant bovine Interleukin-4 and bovine GM-CSF – supplied as a liquid
    • Reconstitution
    • Preparation
      Recombinant cytokines expressed in mammalian Chinese Hamster Ovary (CHO) cells using the pEE14® vector grown in antibiotic free media and USDA-approved dialysed FCS which has been screened for BVDV and virus growth by PCR.
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature this recombinant protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • Acknowledgements
      This reagent was produced as part of the BBSRC/SEERAD Immunological Toolbox. The kit development was also supported by the European Community’s Seventh Framework Programme (FP7, 2007-2013), Research Infrastructures action, under the grant agreement No. FP7-228394 (NADIR)
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays1:20

    • Where this reagent has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
      1. Prepare peripheral blood mononuclear cells (PBMC) from heparinised blood by density gradient centrifugation.

      2. Purify CD14+ve cells by labelling PBMC with CD14 mAb and utilise magnetic bead or flow cytometric separation techniques.

      3. Resuspend the isolated CD14+ve cells at a concentration of 1x106 cells/ml in tissue culture medium (TCM = RPMI or equivalent + 10% foetal calf serum) containing a final dilution of 1:20 of PBP015KZZ .

      4. Add 3ml of cell suspension to each well of a 6 well tissue culture plate.

      5. Culture cells in a humidified atmosphere of 5% CO2 in air, at approximately 37oC.

      6. Culture cells for 3 days. The cells may then be harvested and used for other procedures including immunophenotyping (as required).

      7. If a longer culture period is required the cells must be ‘fed’ with new TCM containing cytokines on day 3:
      Carefully remove 1ml spent medium from each well, care is required to avoid disturbing the cells.
      Add 1.5ml fresh, pre-warmed TCM containing cytokines at 1:20 to each well and re-culture the DC for required culture period (typically up to 7 days).

      8. At the end of the culture period adherent and non-adherent cells can be pooled for use in immunoassays and phenotyped (as required). Adherent cells may require a dissociation step to remove them from the plate.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Bovine Dendritic Cell Growth Kit Formats

    Formats Applications Sizes available
    Bovine Dendritic Cell Growth Kit : Kit FN 5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Human CD14:Alexa Fluor® 647MCA1568A647100 Tests/1mlF
          Mouse anti Human CD14:Alexa Fluor® 700MCA1568A700100 Tests/1mlF
          Mouse anti Human CD14:BiotinMCA1568B0.1 mgF
          Mouse anti Human CD14:FITCMCA1568F0.1 mgF
          Mouse anti Bovine CD14:FITCMCA2678F0.1 mgF
          Mouse anti Bovine MHC Class II DQ:FITCMCA5655F0.1 mgF
          Mouse anti Human CD14:Low EndotoxinMCA1568EL0.5 mgE, F, FN, WB
          Mouse anti Human CD14:Pacific Blue®MCA1568PB100 Tests/1mlF
          Mouse anti Bovine MHC Class II DQMCA56550.25 mgF, IF
          Mouse anti Bovine MHC Class II DRMCA56560.25 mgF
          Mouse anti Bovine CD1w2MCA831G0.25 mgC, F, IP
          Mouse anti Human CD14:RPE-Alexa Fluor® 647MCA1568P647100 Tests/1mlF
          Mouse anti Human CD14:RPEMCA1568PE100 TestsF

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Bovine Dendritic Cell Growth Kit thumbnail image 1


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