Mouse IgG1 Negative Control antibody

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Mouse IgG1 Negative Control Antibody (Human) gallery image 1 — Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse anti Human CD38 (MCA1019A488). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 2 — Figure A. A488 conjugated Mouse anti Human CD38 (MCA1019A488) and RPE-A647 tandem conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. A488 conjugated Mouse anti Human CD38 (MCA1019A488) and RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 3 — Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse anti Human CD11c (MCA2087PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070)

Mouse IgG1 Negative Control Antibody (Human) gallery image 4 — Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD18 (MCA2086PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070).

Mouse IgG1 Negative Control Antibody (Human) gallery image 5 — Figure A. RPE conjugated Mouse anti Human CD18 (MCA2086PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD18 (MCA2086PE) and FITC conjugated Mouse anti Human CD3 (MCA463F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 6 — Figure A. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse IgG1 istoype control (MCA928A647). Figure B. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse anti Human CD4 (MCA1267A647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 7 — Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD46 (MCA2113PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 8 — Figure A. RPE conjugated Mouse anti Human CD46 (MCA2113PE) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. RPE conjugated Mouse anti Human CD46 (MCA2113PE) and APC conjugated Mouse anti Human CD3 (MCA463APC). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 9 — Figure A. RPE conjugated Rat anti Human CD49f (MCA699PE) and A488 conjugated Mouse IgG1 control (MCA928PE). Figure B. RPE conjugated Rat anti Human CD49f (MCA699PE) and A488 conjugated Mouse anti Human CD3 (MCA463A488). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 10 — Figure A. RPE conjugated Mouse anti Human CD59 (MCA1054PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD59 (MCA1054PE) and FITC conjugated Mouse anti Human CD3 (MCA463F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 11 — Image A. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse IgG1 isotype control (MCA928F). Image B. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse anti Human CD177 (MCA2045PE). All experiments performed on human Peripheral blood derived myeloid cells in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 12 — Figure A. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse anti Human CD53 (MCA723F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 13 — Figure A. FITC conjugated Mouse anti Human CD53 (MCA723F) and RPE-A647 tandem conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. FITC conjugated Mouse anti Human CD53 (MCA723F) and RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 14 — Figure A. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD55 (MCA1614PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 15 — Figure A. PE conjugated Mouse anti CD55 (MCA1614PE) and A700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. PE conjugated Mouse anti CD55 (MCA1614PE) and A700 conjugated Mouse anti Human CD3 (MCA463A700). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG1 Negative Control Antibody (Human) gallery image 16 — Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD8a (MCA1223PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 17 — Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD8b (MCA5954PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 18 — Figure A. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 19 — Figure A. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse IgG21 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse anti Porcine CD52 (MCA5974F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 20 — Figure A. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 21 — Figure A. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse anti Porcine CD3 FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 22 — Figure A. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse anti Porcine CD11R1 (MCA1220) detected with Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 23 — Figure A. FITC conjugated Mouse anti Porcine CD18a (MCA1972F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD18a (MCA1972F) and RPE conjugated Mouse anti Porcine CD27 (MCA5973PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 24 — Figure A. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse anti Porcine CD46 (MCA2262) detected with Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 25 — Figure A. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and A647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and A647 conjugated Mouse anti Porcine CD45 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 26 — Figure A. FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F) and APC conjugated Mouse anti Porcine CD31. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 27 — Figure A. APC conjugated Mouse anti Porcine CD31 (MCA1746APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated Mouse anti Porcine CD31 (MCA1746APC) and FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 28 — Figure A. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and PE conjugated Mouse anti Porcine CD16 (MCA1971PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 29 — Figure A. PE conjugated Mouse anti Porcine CD16 (MCA1971PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Porcine CD16 (MCA1971PE) and FITC conjugated Mouse anti Porcine CD45 (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 30 — Figure A. A647 conjugated Mouse anti Porcine CD45 (MCA1222A647) and A488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. A647 conjugated Mouse anti Porcine CD45 (MCA1222A647) and A488 conjugated Mouse anti Porcine CD117 (MCA2598A488). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 31 — Figure A. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse anti Porcine CD163 (MCA2311PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 32 — Figure A. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated Mouse anti Porcine CD45RA (MCA1751F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 33 — Figure A. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 34 — Figure A. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 35 — Figure A. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse anti Canine CD21 (MCA1781PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 36 — Figure A. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse anti Canine CD18 (MCA1780A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 37 — Figure A. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11b (MCA1777S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 38 — Figure A. PE conjugated Rat anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on monocytes.

Mouse IgG1 Negative Control Antibody (Human) gallery image 39 — Figure A. A488 conjugated Rat anti Canine CD4 (MCA1038A488) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. A488 conjugated Rat anti Canine CD4 (MCA1038A488) and purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 40 — Figure A. FITC conjugated Rat anti Canine CD8 (MCA1039F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD8 (MCA1039F) and purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 41 — Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells

Mouse IgG1 Negative Control Antibody (Human) gallery image 42 — Figure A. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA837PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 43 — Figure A. Purified Mouse anti Bovine CD1w3 (MCA1647G) detected with Goat anti Mouse IgG2b RPE (STAR134PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. Purified Mouse anti Bovine CD1w3 (MCA1647G) detected with Goat anti Mouse IgG2b RPE (STAR134PE) and FITC conjugated Mouse anti Bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 44 — Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated Mouse anti Bovine CD8b (MCA1654PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 45 — Figure A. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and purified Mouse IgG1 isotype control (**MCA928**) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). Figure B. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and purified CD5 (MCA835GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 46 — Figure A. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse anti Bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 47 — Figure A. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse anti Bovine CD8b (MCA5779PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 48 — Figure A. FITC conjugated Mouse anti bovine CD62L (MCA1649F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD62L (MCA1649F) and RPE conjugated Mouse anti Bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 49 — Figure A. RPE conjugated Mouse anti Bovine CD80 (MCA2436PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD80 (MCA2436PE) and FITC conjugated Mouse anti Bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 50 — Figure A. FITC conjugated Mouse anti Bovine CD8 (MCA837F) and A647 conjugated Mouse IgG1 isotype control (**MCA928A647**). Figure B. FITC conjugated Mouse anti Bovine CD8 (MCA837F) and A647 conjugated Mouse anti Bovine CD26 (MCA1652A647). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 51 — Figure A. FITC conjugated Mouse anti Bovine CD14 (MCA2678F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated Mouse anti Bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 52 — Figure A. FITC conjugated Mouse anti Bovine CD11b (MCA1425F) and purified Mouse IgG1 isotype control (**MCA928**) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). Figure B. FITC conjugated Mouse anti Bovine CD11b (MCA1425F) and purified CD13 (MCA2338GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 53 — Figure A. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse anti Bovine CD205 (MCA2450PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 54 — Figure A. FITC conjugated Mouse anti Bovine CD282 (HCA151F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD282 (HCA151F) and RPE conjugated Mouse anti Bovine CD32 (MCA5918PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 55 — Figure A. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 56 — Figure A. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**). Figure B. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse anti Bovine CD335 (MCA2365PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG1 Negative Control Antibody (Human) gallery image 57 — Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD25 (MCA2430F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse IgG1 Negative Control

Product Type: Negative/Isotype Control
Isotype: IgG1
Specificity: Mouse IgG1 Negative Control

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References (15)
Applications
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Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.

This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Target Species: Negative Control
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Approx. Protein Concentrations: IgG concentration 0.1mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to that of their test reagents.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood

References for Mouse IgG1 Negative Control antibody

Kupatt, C. et al. (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury.
Arterioscler Thromb Vasc Biol. 20 (10): 2226-32.
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9.
Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9.
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94.
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226.
Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
Pol J Vet Sci. 15 (1): 11-20.
Pakandl, M. et al. (2008) Immune response to rabbit coccidiosis: a comparison between infections with Eimeria flavescens and E. intestinalis.
Folia Parasitol (Praha). 55:1-6.
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