CD3 Antibody | CT-3

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CD3 Antibody | CT-3 gallery image 1

Figure A. RPE conjugated Mouse anti Chicken CD8 (MCA2166PE) and Mouse IgG1 isotype control (MCA928) conjugated with Goat anti Mouse IgG (H/L):FITC (STAR117F). Figure B. Mouse anti Chicken CD3 (MCA1473) conjugated with Goat anti Mouse IgG (H/L):FITC (STAR117F) and RPE conjugated Mouse anti Chicken CD8 (MCA2166PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD3 Antibody | CT-3 gallery image 2

Figure A. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and Mouse IgG1 isotype control (MCA928) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD3 (MCA1473) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD4 (MCA2164F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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CD3 Antibody | CT-3 gallery image 3

Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG1 isotype control (MCA928) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD3 (MCA1473) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.

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  • Mouse anti Chicken CD3
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD3 antibody, clone CT-3, recognizes the chicken CD3 and is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
    • Product Type
      Monoclonal Antibody
    • Clone
      CT-3
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1473C, F, IPdatasheet pdfdatasheet pdf0.5 mg
      MCA1473
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.

        In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
      • Intended Use
      • Target Species
        Chicken
      • Product Form
        Purified IgG - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared from ascites
      • Preservative Stabilisers
        None present
      • Immunogen
        Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Borate buffered saline
      • Fusion Partners
        Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        Please see label for expiry date.
      • GO Terms
        integral to membrane
        transmembrane receptor activity
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/50
        Immunohistology - Frozen
        Immunoprecipitation

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • Flow Cytometry
        Use 50ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : Purified CT-3 C, F, IP 0.5 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                References

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