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CD3 Antibody | CT-3

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  • Mouse anti Chicken CD3
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    CD3 antibody, clone CT-3, recognizes the chicken CD3 and is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1473C, F, IPdatasheet pdfdatasheet pdf0.5 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.

        In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
      • Intended Use
      • Target Species
      • Product Form
        Purified IgG - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared from ascites
      • Preservative Stabilisers
        None present
      • Immunogen
        Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Borate buffered saline
      • Fusion Partners
        Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        Please see label for expiry date.
      • GO Terms
        integral to membrane
        transmembrane receptor activity
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/50
        Immunohistology - Frozen

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • Flow Cytometry
        Use 50ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : Purified CT-3 C, F, IP 0.5 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls


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