CD4 antibody | 2-35
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Mouse anti Chicken CD4:FITC
- Product Type
- Monoclonal Antibody
|Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Turkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Chicken embryonic thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG2b Negative Control:FITC||MCA691F||F||100 Tests||Log in|
|List Price||Your Price|
|Description||Mouse IgG2b Negative Control:FITC|
References for CD4 antibody
Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
Prog Clin Biol Res. 307: 45-56.
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31.
Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
J Immunol. 162 (7): 4115-21.
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57.
Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
Hybridoma. 12: 633-46.
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7.
Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
Avian Dis. 60 (1 Suppl): 262-8.
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73.
View The Latest Product References
Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
Vaccine. 33 (36): 4526-32.
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-19.
Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
Pathogens. 9 (10): 843.
Konieczka, P. et al. (2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
Vet Res. 53 (1): 59.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Chicken Products
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