CD4 antibody | 2-35

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Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 1

Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 2

Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 3

Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 4

Anti Chicken CD4 Antibody, clone 2-35 gallery image 1 — Figure A. RPE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. PE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse anti Chicken CD4 (**MCA2164F**). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.

Anti Chicken CD4 Antibody, clone 2-35 gallery image 2 — Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse anti Chicken CD4 (**MCA2164PE**). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.

Anti Chicken CD4 Antibody, clone 2-35 gallery image 3 — Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG2b isotype control (MCA691) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD4 (**MCA2164GA**) conjugated with Rabbit F(ab')₂ anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.

Anti Chicken CD4 Antibody, clone 2-35 gallery image 4 — **Published customer image:**
FITC conjugated Mouse anti Chicken CD4 antibody, clone 2-35 (**MCA2164F**) used to label turkey lymphocytes by flow cytometry.
**Image caption:**
Gating strategy for extracellular staining for CD4⁺, CD8α⁺, CD4⁺CD8α⁺ and IgM⁺ cells in spleen sample of the examined turkeys. Abbreviations; FSC-A: forward scatter area, FSC-H: forward scatter height SSC-A: side scatter area.

From: Konieczka P, Tykałowski B, Ognik K, Kinsner M, Szkopek D, Wójcik M, Mikulski D, Jankowski J.
Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
Vet Res. 2022 Jul 26;53(1):59.
doi: 10.1186/s13567-022-01080-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Chicken CD4:FITC

Product Type: Monoclonal Antibody
Clone: 2-35
Isotype: IgG2b
Specificity: CD4

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References (12)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2164F Datasheet Datasheet SDS SDS	F	0.1 mg		Log in	Get Quote
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Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).

Target Species: Chicken
Species Cross-Reactivity: Target Species Cross Reactivity

Turkey; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Chicken embryonic thymocytes
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Turkey

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG2b Negative Control:FITC	MCA691F	F	100 Tests	Log in	Get Quote
List Price	Your Price
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Description	Mouse IgG2b Negative Control:FITC

References for CD4 antibody

Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
Prog Clin Biol Res. 307: 45-56.
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
Poult Sci. 78 (11): 1526-31.
Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
J Immunol. 162 (7): 4115-21.
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57.
Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
Hybridoma. 12: 633-46.
Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
Arch Virol. 159 (6): 1453-7.
Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
Avian Dis. 60 (1 Suppl): 262-8.
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
Poult Sci. 96 (7): 2064-73.

View The Latest Product References

Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
Vaccine. 33 (36): 4526-32.
Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
Int J Curr Microbiol App Sci. 6 (1): 15-19.
Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
Pathogens. 9 (10): 843.
Konieczka, P. et al. (2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
Vet Res. 53 (1): 59.