Pertuzumab antibody | AbD37069ia
Human anti Pertuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD37069ia
- Isotype
- IgG1
- Specificity
- Pertuzumab
Human Anti-Pertuzumab Antibody, clone AbD37069ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody pertuzumab. It does not recognize free human epidermal growth factor receptor 2 (HER2) nor pertuzumab in complex with HER2. The antibody can be used to measure free pertuzumab or its biosimilars in patient samples. Clone AbD37069ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 2 nM, and is one of two antibodies with different affinities offered for ADA assays. Clone AbD37069ia is also suitable as a detection antibody for pertuzumab in a pharmacokinetic (PK) bridging ELISA to measure free drug when paired with clone AbD37070 (HCA342, Fab-FH format) as the capture reagent. Pertuzumab (Perjeta) is an IgG1/kappa antibody humanized from mouse. It has been approved for the treatment of patients with metastatic HER2-positive breast cancer in combination with trastuzumab. Pertuzumab binds to the extracellular domain II of HER2 and inhibits HER2–HER3 dimerization, reducing signaling through intracellular pathways (Capelan et al. 2013). View a summary of all anti-pertuzumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Pertuzumab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Pertuzumab antibody, clone AbD37069ia is KD = 2 nM as measured by real time, label free molecular interaction analysis on immobilized Pertuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Perjeta is a trademark of Genentech, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Pertuzumab antibody, clone AbD37069ia can be used in a direct or an indirect ELISA or as detection antibody for Pertuzumab in a bridging ELISA together with HCA342 (AbD37070, Fab-FH) as the capture reagent. It can also be used as a calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA.
Protocol: PK bridging ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Hispec Assay Diluent | ||||||
Human anti Pertuzumab | HCA341 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Pertuzumab | ||||||
Human anti Pertuzumab | HCA342 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Pertuzumab | ||||||
Human anti Pertuzumab | HCA344 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Pertuzumab | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | LYNX Rapid HRP Antibody Conjugation Kit |
Further Reading
-
Capelan, M. et al. (2013) Pertuzumab: new hope for patients with HER2-positive breast cancer.
Ann Oncol. 24 (2): 273-282.
- Synonyms
- Perjeta
HCA345
159800 162606 162607 168159If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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