Pertuzumab antibody | AbD37069ia
Human anti Pertuzumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD37069ia
- Isotype
- IgG1
- Specificity
- Pertuzumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human anti pertuzumab antibody, clone AbD37069ia (HCA345P) at a concentration of 2μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with human HER2, pertuzumab, trastuzumab, human IgG1 kappa or lambda at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, the human HER2/pertuzumab complex was formed by adding 5μg/ml pertuzumab to the wells coated with human HER2. Detection was performed using HRP conjugated Human anti pertuzumab antibody, clone AbD37069ia (HCA345P) titrated to the given concentrations in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data for Pertuzumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human anti pertuzumab antibody, clone AbD37070 (HCA342) at a concentration of 1μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of pertuzumab. Detection was performed using HRP conjugated Human anti pertuzumab antibody, clone AbD37069ia (HCA345P) at a concentration of 2μg/ml in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with HER2 at a concentration of 1μg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of pertuzumab (0.15μg/ml) plus increasing concentrations of Human anti pertuzumab antibody, clone AbD37069ia (HCA345) in the monovalent Fab format was added. Free pertuzumab, still capable of binding to the HER2 coated plate, was detected using an HRP conjugated Mouse Anti-Human IgG (Fc) CH2 domain antibody, clone MK 1 A6 (MCA647P) followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with pertuzumab at a concentration of 1μg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human anti pertuzumab antibody, clone AbD35668ia (HCA344, black), or clone AbD37069ia (HCA345, red). Detection was performed using HRP conjugated pertuzumab followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
Filter by Application:
E Reset| Human Anti-Pertuzumab Antibody, clone AbD37069ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody pertuzumab. It does not recognize free human epidermal growth factor receptor 2 (HER2) nor pertuzumab in complex with HER2. The antibody can be used to measure free pertuzumab or its biosimilars in patient samples. Clone AbD37069ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 2 nM, and is one of two antibodies with different affinities offered for ADA assays. Clone AbD37069ia is also suitable as a detection antibody for pertuzumab in a pharmacokinetic (PK) bridging ELISA to measure free drug when paired with clone AbD37070 (HCA342, Fab-FH format) as the capture reagent. Pertuzumab (Perjeta) is an IgG1/kappa antibody humanized from mouse. It has been approved for the treatment of patients with metastatic HER2-positive breast cancer in combination with trastuzumab. Pertuzumab binds to the extracellular domain II of HER2 and inhibits HER2–HER3 dimerization, reducing signaling through intracellular pathways (Capelan et al. 2013). View a summary of all anti-pertuzumab antibodies |
|
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Pertuzumab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Pertuzumab antibosy, clone AbD37069ia is KD = 2 nM as measured by real time, label free molecular interaction analysis on immobilized Pertuzumab.
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Perjeta is a trademark of Genentech, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This antibody can be used in a direct ELISA or as detection antibody for Pertuzumab in a bridging ELISA together with HCA342 (AbD37070, Fab-FH) as the capture reagent.
Protocol: PK bridging ELISA.
Further Reading
-
Capelan, M. et al. (2013) Pertuzumab: new hope for patients with HER2-positive breast cancer.
Ann Oncol. 24 (2): 273-282.
- Synonyms
- Perjeta
HCA345P
169324
169336
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with PERTUZUMAB specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up
