Tubulin Alpha Antibody | YL1/2

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Tubulin Alpha Antibody | YL1/2 gallery image 1

HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP (MCA77P)

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Tubulin Alpha Antibody | YL1/2 gallery image 2

Western blot analysis of C6 rat glioma whole cell lysate probed with Rat anti tubulin alpha antibody (MCA77G) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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Tubulin Alpha Antibody | YL1/2 gallery image 3

Published customer image:
Rat anti Tubulin alpha antibody, clone YL1/2 used for detection of cytoskeleton in T. brucei.
Image caption:
TbRRM1 deficient cells exhibited a nozzle phenotype. (A) Upper: Graphic bars showing the length from nucleus to kinetoplast (N-K; gray bars) or from kinetoplast to posterior end (K-P; black bars) at 0, 24, 48 and 72 h post RNAi induction. Results are expressed as mean ± standard deviation from more than 200 cells at each time point of three independent experiments (Student's t test. *p<0,05; **p<0,01). Bottom: DAPI stained PC. (B) Cytoskeleton analysis of 48 h TbRRM1 silenced cells by immunofluorescence (anti-tyrosinated tubulin YL1/2). From left to right: phase contrast, DAPI stained cells, YL1/2 stained cells and merged images. Scale bar: 3 µm.

From: Levy GV, Bañuelos CP, Níttolo AG, Ortiz GE, Mendiondo N, Moretti G, et al. (2015) Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei.
PLoS ONE 10(8): e0136070.

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Published customer image:
Rat anti tubulin-α antibody, clone YL1/2 used for the detection of cytoskeleal elements in drosophila embryos by immunofluorescence.
Image caption:
Spindle abnormalities in embryos derived from imp-α2D14/imp-βKetRE34 and imp-α2D14/imp-βc02473; NLSB-/+ females. (A–D) Wild-type and mutant embryos stained for α-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-α2D14/imp-βKetRE34 and (C) imp-α2D14/imp-βc02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 μm. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.

From: Virágh E, Gorjánácz M, Török I, Eichhorn T, Kallakuri S, Szlanka T, Kiss I, Mechler BM. Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions. G3 (Bethesda). 2012 Jan;2(1):1-14.

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Published customer image:
Rat anti tubulin-αused as a loading control for cell lysates using western blotting.
Image caption:
Direct detection of the M42 polypeptide. (A) Validation of anti-M42 serum. Lysates from cells transfected with the indicated GFP polypeptides were analysed by SDS-PAGE and western blotting as labeled. (B) Detection of M42 from virus-infected cells. Lysates from cells infected with the indicated viruses at 10 h p.i. were analysed by SDS-PAGE and western blotting as labeled. The same membrane was probed with mouse anti-M2 14C2 and rabbit anti-M42 using different colour secondary antisera; individual grey scale and colour merged images are shown.

From: Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, et al. (2012) Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain. PLoS Pathog 8(11): e1002998.

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Published customer image:
Rat anti tubulin-α antibody, clone YL1/2 used for detection of tubulin in the ctenophore Pleurobrachia pileus
Image caption:
Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A’) The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A”) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A’). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 μm; D-F, I-K, N-P: 200 μm; Q: 100 μm; R, S: 10 μm; T: 50 μm; U, V, W: 25 μm.

From: Dayraud C, Alié A, Jager M, Chang P, Le Guyader H, Manuel M, Quéinnec E. Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective.
BMC Evol Biol. 2012 Jul 2;12:107.

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Rat anti tubulin-α antibody, clone YL1/2 used for the detection of tubulin expression in the cnidarian Clytia hemisphaerica by immunofluorescence.
Image caption:
Distribution of the nerve net in two-day-old planula detected using YL1/2 antibody. (A) Superficial view (optical plane on the basal part of the ectodermal epithelium). (B) Deeper view of the same specimen (optical plane crossing the larval endoderm and cavity). (C) Higher magnification view of the YL1/2 staining (in red) with Dapi counter-staining (in blue) showing the distribution and aspect of nerve cell bodies (white arrowheads) and neurites. Oral pole is on the top for all pictures. Scale bars: A-B, 50 μm; C, 10 μm.

From: Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica Muriel Jager, Eric Quéinnec, Hervé Le Guyader and Michaël Manuel. EvoDevo 2011, 2:12.

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Tubulin Alpha Antibody | YL1/2 gallery image 8

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Rat anti Tubulin-α antibody, clone YL1/2 (MCA77G) used as a loading control for protein loading in western blotting experiments.
Image caption:
An FHA mutation suppresses Tel1 activity but not Mec1 activity during both mitosis and meiosis.
A. Phosphorylation of Rad53 protein was analyzed at the indicated time points after phleomycin addition. Rad53 protein was detected by Western blotting by using anti-Rad53 in wild type (W303-1A), xrs2-SH (MSY2199), rad50S (MSY2203), rad50S mec1Δ (MSY2461) and mec1Δ rad50S xrs2-SH (MSY2372). Asterisks indicate phosphorylation signal of Rad53. Tubulin blots are shown as internal control for protein loading. B. Meiosis progression at the indicated time points after SPM transition in wild type (NKY1551), rad50S (MSY1758), xrs2-SH (MSY1867), rad50S xrs2-SH (MSY1844), xrs2-664 (MSY2015), rad50S xrs2-664 (MSY2106) and rad50S tel1Δ (MSY1952). The percentage of cells containing two or more nuclei per ascus (post-MI%) was plotted in the graphs. Images to the right show typical DAPI images after 6 hr of meiosis in each cell line, and the corresponding percentage of post-MI cells for each strain is shown. Scale bar indicates 2 μm. C. Meiosis progression in wild type (NKY1551), xrs2-SH (MSY1867), dmc1Δ (MSY2638) and dmc1Δ xrs2-SH (MSY4674). Typical DAPI micrographs after 6 hr of meiosis in each cell line, with addition of mec1Δ dmc1Δ as a control, are shown to the right. Scale bar indicates 2 μm. D. Phosphorylation of Hop1 at T318 (pT318) at the indicated time points after SPM transition was analyzed in wild type (NKY1551), rad50S (MSY1758), xrs2-SH (MSY1867), rad50S xrs2-SH (MSY1844), xrs2-664 (MSY2015), rad50S xrs2-664 (MSY2106) and rad50S tel1Δ (MSY1952). Hop1 protein was analyzed by western blot with anti-Hop1 (Hop1; green) or with anti-Hop1-pT318 antibody (pT318; magenta), and then each fluorescence signal was detected by laser scanner on the same membrane. Highly mobility shifted band was marked with asterisk. A merged image is shown on the top. Tubulin blots are shown as an internal control for protein loading. E. Localization of Hop1 (red) and Hop1-pT318 (green) on the meiotic nuclear spreads at 4 hr after SPM transition was analyzed in wild type (NKY1551), rad50S (MSY1758), xrs2-SH (MSY1867), rad50S xrs2-SH (MSY1844), xrs2-664 (MSY2015), rad50S xrs2-664 (MSY2106) and rad50S tel1Δ (MSY1952). Scale bar indicates 2 μm.

From: Iwasaki D, Hayashihara K, Shima H, Higashide M, Terasawa M, Gasser SM, et al. (2016)
The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA–Dependent Tel1 Activation.
PLoS Genet 12(3): e1005942.

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Tubulin Alpha Antibody | YL1/2 gallery image 9

Published customer image:
Rat anti apha-tubulin antibody, clone YL1/2 (MCA77G) used for the detection of tyrosinated tubulin expressing cells in rat neurons by immunofluorescence.
Image caption:
Increased microtubule growth in GSK3β depleted neurons is related to decreased CRMP-2 phosphorylation. (A) MAP1B, CLASP2 and CRMP-2 western in CAD cells. (B) Microtubule growth in rat neurons transfected with EB3-mCherry alone (control) or co-transfected with WT MAP1B, ST/AA-MAP1B or ST/DD-MAP1B. (C) Intensity ratios between acetylated and tyrosinated microtubules in growth cones from rat DRG neurons transfected with EB3-mCherry alone (control) or co-transfected with WT MAP1B, ST/AA-MAP1B or ST/DD-MAP1B. (D) Representative images of C. Scale bar: 3 μm. (E) Intensity ratios between acetylated and tyrosinated microtubules in growth cones from rat naïve DRG neurons either non-infected (control) or infected with WT CLASP2, 9S/A-CLASP2 or 8S/D-CLASP2. (F) Representative images of E. Scale bar: 3 μm. (G) Microtubule growth in neurons transfected with EB3-GFP alone (control) or co-transfected with WT CRMP-2, T/A-CRMP-2, or T/D-CRMP-2. (H) Intensity ratios between acetylated and tyrosinated microtubules in growth cones from neurons transfected with EB3-GFP alone (control) or co-transfected with WT CRMP-2, T/A-CRMP-2, or T/D-CRMP-2. (I) Representative images of H. Scale bar: 5 μm. (J) Microtubule growth in naïve neurons from cre+GSK3βwt/wt mice or cre+GSK3βlox/lox mice transfected with EB3-mCherry, or cre+GSK3ßlox/lox mice co-transfected with EB3-mCherry and T/D-CRMP-2 (cre+GSK3βlox/lox + T/D-CRMP-2). (K) Intensity ratios between acetylated and tyrosinated microtubules in growth cones from cre+GSK3βwt/wt, cre+GSK3βlox/lox or cre+GSK3βlox/lox transfected with T/D-CRMP-2 (cre+GSK3βlox/lox + T/D-CRMP-2). (L) Representative images of K. Scale bar: 5 μm. All error bars are SEM. *P <0.05. **P <0.01. ***P <0.001. ****P <0.0001. Two-tailed Student's t test. CLASP2, cytoplasmic linker associated protein 2; CRMP, collapsin response mediator protein; DRG, dorsal root ganglia; GSK3β, glycogen synthase kinase 3β; MAP1B, microtubule-associated protein 1B; SEM, standard error of the mean. WT, wild type.

From: Liz MA, Mar FM, Santos TE, Pimentel HI, Marques AM, Morgado MM, Vieira S, Sousa VF, Pemble H, Wittmann T, Sutherland C, Woodgett JR, Sousa MM.
Neuronal deletion of GSK3β increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2.
BMC Biol. 2014 Jun 12;12:47.

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  • Rat anti Tubulin Alpha:DyLight®750
  • Rat anti Tubulin Alpha:HRP
  • Rat anti Tubulin Alpha:DyLight®800
  • Rat anti Tubulin Alpha
  • Rat anti Tubulin Alpha:DyLight®680
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YL1/2
  • Isotype
    IgG2a
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA77D750WBdatasheet pdfdatasheet pdf0.1 mg
    MCA77D750
    MCA77PC, E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA77P
    MCA77D800WBdatasheet pdfdatasheet pdf0.1 mg
    MCA77D800
    MCA77D680WBdatasheet pdfdatasheet pdf0.1 mg
    MCA77D680
    MCA77GC, E, IF, IP, R, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA77G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

      The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).

      This product is routinely tested in ELISA on Tubulin.
    • Intended Use
    • Target Species
      Yeast
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ashbyayes
      BirdsExpected from Sequence
      EchinodermExpected from Sequence
      Humanyes
      Mouseyes
      Dogyes
      Nephrotoma suturalis no
      Hemicentrotus pulcherrimusyes
      Potatoyes
      Bombyx moriyes
      Rhodnius prolixusyes
      Fungalno
      Arabidopsisyes
      Strongylocentrotus purpuratusyes
      Dendraster excentricusyes
      Trypanosoma bruceiyes
      Potorous tridactylisyes
      Bovineyes
      Pleurobrachiayes
      Caenorhabditisyes
      Dictyostelium discoideumyes
      Xenopusyes
      Pig-tailed macaqueyes
      Clytia sp.yes
      Ratyes
      Pigyes
      Drosophilayes
      PlantsExpected from Sequence
      AmphibiaExpected from Sequence
      Saccharomycesyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to DyLight®750 - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to DyLight®800 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to DyLight®680 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Yeast tubulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Radioimmunoassays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA77D750 is suitable for use as a loading control.
    • Western Blotting
      MCA77P is suitable for use as a loading control.
    • Western Blotting
      MCA77D800 is suitable for use as a loading control.
    • Western Blotting
      MCA77G is suitable for use as a loading control.
    • Western Blotting
      MCA77D680 is suitable for use as a loading control.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Tubulin Alpha Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Alpha Antibody : DyLight®680 YL1/2 WB 0.1 mg
    Tubulin Alpha Antibody : HRP YL1/2 C, E, WB 0.1 mg
    Tubulin Alpha Antibody : DyLight®750 YL1/2 WB 0.1 mg
    Tubulin Alpha Antibody : DyLight®800 YL1/2 WB 0.1 mg
    Tubulin Alpha Antibody : Purified YL1/2 C, E, IF, IP, R, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
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      STAR72
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      STAR21B
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      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

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