Complete Solutions for your Western Blotting Workflow
Explore Bio-Rad's complete solutions for your western blotting workflow — gels, electrophoresis chambers, western blotting transfer and imaging systems, buffers, membranes, and immunodetection reagents and kits.
PrecisionAb™ Lab-Validated Antibodies for Western Blotting
These antibodies are fully lab-validated using strict testing criteria to deliver superior performance in western blotting detection.
Western blotting, the transfer of proteins to a solid-phase membrane support followed by immunodetection, is a powerful and popular technique for the visualization and identification of proteins. Western blotting combines the resolution of gel electrophoresis with the specificity of immunoassays, allowing the identification and analysis of individual proteins in complex samples.
With over 25 years of western blotting expertise, Bio-Rad provides a wealth of information and products to help optimize and troubleshoot western blotting. This section taps into this expertise, providing an overview of western blotting methods and help with troubleshooting as well as information about products and solutions that will help you obtain the highest-quality western blotting data.
The western blotting workflow involves the selection of appropriate methods, apparatus, membrane, buffer, and transfer conditions. This involves two phases: protein transfer to a membrane and detection of the membrane-immobilized protein. Once proteins are immobilized on a membrane, they are available for visualization, detection, and analysis.
Learn more about western blotting techniques
Are you struggling with western blots? The Western Blot Doctor™ is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.
Visit the Western Blot Doctor
Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol.
The V3 Western Workflow utilizes Bio-Rad’s proprietary stain-free technology to provide visual checkpoints at each step of the western blotting protocol and an alternative to the use of housekeeping proteins via total protein normalization, eliminating stripping and reprobing and delivering high-quality results in half the time.
Learn more about the V3 Western Workflow
Bio-Rad’s western blotting products include the V3 Western Workflow, systems for protein transfers, blotting membranes, filter paper, premixed blotting buffers and reagents, protein standards, and detection kits.
View all western blotting products on Bio-Rad.com
Bio-Rad’s TGX™ Gel chemistry enables high resolution separation in only 15–20 min.
Efficient and reproducible transfer of proteins up to 400 kD in as little as 3 min.
Sensitivity and resolution equal to film with superior signal-to-noise ratio and dynamic range.
Clarity Western ECL Substrate gives femtogram-level sensitivity and long signal with any HRP-conjugate secondary antibody.
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