"A question of Life or Death" - differentiating between healthy and apoptotic cells
Dead or Alive on the ZE5™ - Using flow cytometry to measure apoptosis
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Figure 1. A mixture of live and heat-killed Jurkat cells were stained with ReadiDrop 7-AAD for 1 min prior to sorting
The ReadiDrop 7-aminoactinomycin D (7-AAD) cell viability dye is designed as a ready-to-use 7-AAD solution, to exclude dead cells in Flow Cytometry and Immunofluorescence Microscopy. 7-AAD enters a cell with a compromised membrane and binds to double-stranded DNA.
This dye is available as a convenient ready-to-use solution: simply add 1 or 2 drops of the 7-AAD solution to assess the viability of your cells.
Figure 2. Excitation and emission spectra of 7-AAD. Maximal excitation is 546 nm (blue) and maximal emission is 647 nm (red).
The ReadiDrop 7-AAD cell viability dye has a number of key benefits and these include:
Key dye characteristics and handling instructions for ReadiDrop 7-AAD cell viability dye include:
We also offer fixable cell viability dyes in a range of excitation and emission spectra to incorporate into multi-color Flow Cytometry panels.