Propidium Iodide - Cell Viability Dye

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Determine your Cell Viability with 1-2 drops

Figure 1. A mixture of live and heat-killed Jurkat cells were stained with ReadiDrop propidium iodide for 1 min prior to sorting.

Readidrop Propidium iodide (PI) cell viability dye is used in Flow Cytometry to exclude dead cells by entering a cell with a compromised membrane and binding to double-stranded DNA and RNA.

It is available as a convenient ready-to-use solution: simply add 1 or 2 drops of the propidium iodide solution to assess the viability of your cells. Suitable for use in Flow Cytometry and Immunofluorescence Microscopy.

Propidium Iodide Cell Viability Assay Key Benefits:

The ReadiDrop propidium iodide cell viability assay has a number of key benefits and these include:

• Ideal for Flow Cytometry and Cell Corting - PI is in a PBS solution without preservative
• Easy storage: as it is stable at room temperature you can  keep a bottle of ReadiDrop propidium iodide cell viability assay by your cell sorter or cell analyzer for easy access
• No additional preparation - 1 µg/ml final concentration when 1-2 drops added to your cells

PI Dye Spectrum and Features

Figure 2. Excitation and emission spectra. Excitation and emission spectra of PI. Maximal excitation is 535 nm (blue) and maximal emission is 617 nm (red).(red).

Key characteristics and handling instructions of the ReadiDrop propidium iodide cell viability dye are:

Fluorophore Properties

• Max excitation: 535 nm
• Max emission: 617 nm
• Optimal excitation laser: 561 nm or 488 nm

Molecular Properties

• Molecular weight: 668.39 g/mol
• Molecular formula: C₂₇H₃₂I₂N₄

Chemical structure

Specifications

• Store at 15–30°C
• Do not freeze

We also offer fixable viability dyes in a range of excitation and emission spectra to enable easy incorporation into multi-color Flow Cytometry panels

View our fixable viability dye range