"A question of Life or Death" - differentiating between healthy and apoptotic cells
Dead or Alive on the ZE5™ - Using flow cytometry to measure apoptosis
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Figure 1. A mixture of live and heat-killed Jurkat cells were stained with ReadiDrop propidium iodide for 1 min prior to sorting.
Propidium iodide (PI) cell viability dye is used in Flow Cytometry to exclude dead cells by entering a cell with a compromised membrane and binding to double-stranded DNA and RNA.
It is available as a convenient ready-to-use solution: simply add 1 or 2 drops of the propidium iodide solution to assess the viability of your cells. Suitable for use in Flow Cytometry and Immunofluorescence Microscopy.
Figure 2. Excitation and emission spectra. Excitation and emission spectra of PI. Maximal excitation is 535 nm (blue) and maximal emission is 617 nm (red).(red).
The ReadiDrop propidium iodide cell viability assay has a number of key benefits and these include:
Key characteristics and handling instructions of the ReadiDrop propidium iodide cell viability dye are:
We also offer fixable viability dyes in a range of excitation and emission spectra to enable easy incorporation into multi-color Flow Cytometry panels