DYKDDDDK Tag Antibody

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  • Rabbit anti DYKDDDDK Tag
  • Rabbit anti DYKDDDDK Tag:HRP
  • Rabbit anti DYKDDDDK Tag
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1074PE, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1074P
    AHP1074GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1074GA
    AHP1074E, WBdatasheet pdfdatasheet pdf50 µg
    AHP1074
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.

      Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.

      In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody has been shown not to cross-react with any endogenous proteins.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 0.1ml deionised water. For long term storage at -20oC, add glycerol to a final concentration of 50%.
    • Preparation
    • Antiserum Preparation
      Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      Pack Size: 0.1 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      0.01% Gentamicin Sulfate
      1% Bovine Serum Albumin
      Pack Size: 0.1 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
    • Immunogen
      Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
      Pack Size: 50 µgIgG concentration 0.5mg/ml
      IgG concentration 1mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
      Pack Size: 50 µgIgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/90001/450000
      Western Blotting1/20001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/50,000
      Western Blotting1/1,0001/5,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/90001/450000
      Western Blotting1/20001/10000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional DYKDDDDK Tag Antibody Formats

    Formats Applications Sizes available
    DYKDDDDK Tag Antibody : Purified E, WB 50 µg | 0.1 mg
    DYKDDDDK Tag Antibody : HRP E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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