IL-8 Antibody | 8M6

IL-8 Antibody | 8M6 gallery image 1

Published customer image:
Mouse antiSheep interleukin-8 antbody, clone 8M6 (MCA1660) used, in conjunction with Rabbit anti Sheep interleuin-8 antibody (AHP425 ) for the measurement of ovine CXCL8 in embryonal bovine lungs by ELISA.
Image caption:
Analysis of bovine TLR5 activity in bovine epithelial cells.
The bovine EBL cell line was stably transfected with a bTLR5 clone and CXCL8 secretion assayed following challenge with flagellin. (A) CXCL8 levels from EBLs with and without transfection of bTLR5. H7 flagellin at a range of concentrations was added to the cells and CXCL8 levels were measured by ELISA following overnight incubation. Transfection with bTLR5 resulted in significantly higher levels of CXCL8 being produced by the bTLR5+ cells with addition of 50-50,000 ng/mL of H7 flagellin (p?<?0.001). The data shown are the means and 95% confidence intervals. (B) Analysis of CXCL8 production from EBL-TLR5 in response to addition of native H7 or an H7 flagellin preparation from which the majority of LPS has been removed. Medians and interquartile ranges are shown. (C) Secreted CXCL8 following addition of WT and mutated H7 flagellins to EBLs with transfected bTLR5. 50 ng/mL of WT-H7 and mutated flagellins were added to the EBLs transfected with bTLR5 and incubated overnight. CXCL8 was measured by ELISA. Addition of the R90T, R90A and L500A/I504A variants led to significantly lower levels of cytokine release (p?<0.001) relative to WT-H7 stimulation (asterisks). R90T showed a significantly lower induction than R90A (p?<0.001). Medians and interquartile ranges are shown. (D) Secreted CXCL8 following addition of WT H7 and the R90T flagellin mutant to EBL cells. A range of flagellin concentrations was incubated overnight with the cells and CXCL8 measured by ELISA. R90T flagellin demonstrated significantly reduced levels of CXCL8 activation at 50 ng/mL and 500 ng/mL (p?<0.001), marked by asterisks. The data shown are the means and 95% confidence intervals. All CXCL8 data shown is from a minimum of three biological replicates.

From: Tahoun A, et al.
Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 2015 Feb 19;46:9.

IL-8 Antibody | 8M6 gallery image 2

Published customer image:
Mouse anti Sheep interleukin-8 antibody, clone 8M6 used for the identification of IL-8 expressing porcine vaginal epithelial cells in a chlamydial infection model by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
IHC staining of cox-2 and IL-8 in vaginal tissue from day 3 PI, and control tissues. a Vagina, day 3 PI. Positive staining in numerous superficial epithelial cells for cox-2 (arrows); b Vagina from control tissue, showing positive staining for cox-2 in single superficial epithelial cells (arrow); c Vagina, day 3 PI. Positive (red) staining in numerous superficial epithelial cells for IL-8 (arrows); d Vagina from control tissue showing positive staining for IL-8 in a single epithelial cell (arrow).

From: Erneholm K, Lorenzen E, B7oslash;je S, Olsen AW, Andersen P, Cassidy JP, Follmann F, Jensen HE, Agerholm JS.
Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D. BMC Vet Res. 2016 Sep 10;12(1):200.

  • IL-8 Antibody | 8M6 thumbnail image 1
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  • Mouse anti Sheep Interleukin-8
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1660E, F*, FN*, WBdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Mouse anti Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.

      Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causitive agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exagerrated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndon et al. 2010).

      Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursen et al. 2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8.
      Mouse anti Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measuse IL-8 concentrations in bovine samples (Cronin et al. 2015).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Flow Cytometry(1)1/10
      Functional Assays(2)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm&trade: (Product Code BUF09A) for this purpose.
      Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      Mouse anti Sheep Interleukin-8 antibody, clone 8M6 may be used in combination with AHP425 in sandwich ELISA assays for ovine IL-8.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-8 Antibody Formats

    Formats Clone Applications Sizes available
    IL-8 Antibody : Purified 8M6 E, F*, FN*, WB 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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