IgA Antibody

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IgA Antibody gallery image 1

Published customer image:
Rabbit anti Sheep IgA antibody used as a capture reagent (AHP949) together with horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) as a detection reagent for the measurement of total IgA in sheep saliva using a sandwich ELISA.
Image caption:
Stability of total sheep IgA (A) and IgG (B) (n = 15), rGT2-specific IgA (C) and IgG (D) (n = 10) exposed to rumen contents from sheep. Saliva was added to rumen contents with or without addition of protease inhibitor or added to PBS. Ig concentrations were measured by ELISA. Data are presented as mean (± SE) proportion of antibody relative to levels at time 0. Significant differences to time 0 were shown as *P <0.05, **P <0.001.

From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016)
Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
PLoS ONE 11(7): e0159861.

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IgA Antibody gallery image 2

Published customer image:
Horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) used as a detection reagent for the measurement of total IgA in sheep saliva by western blotting.
Image caption:
Western blot analysis showing the stability of sheep IgA (left) and IgG (right) before (0 h) and after (4 h) incubation with sheep rumen contents or PBS at 39°C. Lane 1: standards of different molecular weights; lane 2: rumen contents (0 h); lane 3: rumen contents (4 h); lane 4: rumen contents with protease inhibitor (0 h); lane 5: rumen contents with protease inhibitor (4 h); lane 6: PBS (0 h) and lane 7: PBS (4 h).

From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016)
Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
PLoS ONE 11(7): e0159861.

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IgA Antibody gallery image 3

Published customer image:
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Scatterplots showing relationships among the four fecal antibody measures (“anti-Tc”: anti-T. circumcincta antibodies). X-axis and y-axis units represent optical densities at a wavelength of 450 nm.

From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.

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IgA Antibody gallery image 4

Published customer image:
Relationship between fecal and plasma antibody measurements.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Scatterplots showing relationships between the four fecal antibody measures and the five plasma antibody measures (“anti-Tc”: anti-T. circumcincta antibodies), with points in red where the correlation was statistically significant. X-axis and y-axis units represent optical densities at a wavelength of 450 nm.

From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.

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IgA Antibody gallery image 5

Published customer image:
Antibody response by age and sex group.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Variation in the four fecal and five plasma antibody measures with respect to age and sex group. Each data point is represented by a black dot. For box plots, the bar in centre of box represents the median value, the box represents the interquartile range, and bars indicate the 5–95th percentile range. fem, female.

From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.

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IgA Antibody gallery image 6

Published customer image:
Antibody response by Fecal egg count according to age and weight.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Variation in weight (A) and log-transformed strongyle fecal egg count (FEC) with respect to age and sex groups, with the mean and SEM indicted by the bars. (C, D): Scatterplot illustrating the significant negative correlation between FEC and fecal anti-T. circumcincta IgA and IgG antibody levels, respectively.

From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.

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  • IgA Antibody thumbnail image 1
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  • Rabbit anti Sheep IgA
  • Rabbit anti Sheep IgA:FITC
  • Rabbit anti Sheep IgA:HRP
  • Rabbit anti Sheep IgA:Alk. Phos.
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP949ABEdatasheet pdfdatasheet pdf0.5 mg
    AHP949AB
    AHP949Edatasheet pdfdatasheet pdf1 mg
    AHP949
    AHP949FFdatasheet pdfdatasheet pdf1 mg
    AHP949F
    AHP949PEdatasheet pdfdatasheet pdf1 mg
    AHP949P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti sheep IgA antibody recognizes ovine IgA. This antibody has been shown to react specifically with ovine IgA by immunoelectrophoresis and ELISA.
      Rabbit anti sheep IgA antibody may cross react with IgA from other species.
    • Intended Use
    • Target Species
      Sheep
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ovine IgA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/2001/2000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : HRP E 1 mg
    IgA Antibody : Purified E 1 mg
    IgA Antibody : Alk. Phos. E 0.5 mg
    IgA Antibody : FITC F 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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