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Serotonin antibody | YC5/45

Rat anti Serotonin

Product Type
Monoclonal Antibody
Clone
YC5/45
Isotype
IgG2
Specificity
Serotonin

Product Code Applications Pack Size List Price Your Price Qty
8250-0495
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * IF 0.1 ml loader
List Price Your Price
loader

Rat anti serotonin antibody, clone YC5/45 recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.

Serotonin, also known as 5-hydroxytryptamine or 5-HT is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.

Serotonin appears widely conserved and Rat anti serotonin antibody, clone YC5/45 has been used successfully to demonstrate serotonin immunoreactivity using immunohistochemistry in many diverse groups including primates (Vanoli et al. 2013), rodents (Hosoda et al. 1984), prototherian mammals, avians and reptiles (Adamson and Campbell 1988), teleosts (Takeda et al. 2014) through to invertebrates including hirudinids (Masuda-Nakagawa et al. 1990) and gastropods (Osborne and Dockray 1982).

Target Species
Broad
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Goldfish
Cat
Chicken
Marine file snake
Helix aspersa
Hirudo medicinalis
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Serotonin conjugated to bovine serum albumin.
Fusion Partners
Spleen cells from immunized COB wistar rats were fused with cells of the Y3-Ag-1.2.3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1
  1. 1Formaldehyde fixation is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Source Reference

  1. Consolazione, A. et al. (1981) Immunocytochemical detection of serotonin with monoclonal antibodies.
    J Histochem Cytochem. 29: 1425-30.

References for Serotonin antibody

  1. Osborne, N.N. (1982) Uptake, localization and release of serotonin in the chick retina.
    J Physiol. 331: 469-79.
  2. Cuello, A.C. et al. (1982) Immunocytochemistry with internally labeled monoclonal antibodies.
    Proc Natl Acad Sci U S A. 79: 665-9.
  3. Milstein, C. et al. (1983) The discrepancy between the cross-reactivity of a monoclonal antibody to serotonin and its immunohistochemical specificity.
    Mol Immunol. 20 (1): 113-23.
  4. Suárez-Quian, C.A. (1987) The distribution of four lysosomal integral membrane proteins (LIMPs) in rat basophilic leukemia cells.
    Tissue Cell. 19: 495-504.
  5. Solcia, E. et al. (1990) Gastric argyrophil carcinoidosis in patients with Zollinger-Ellison syndrome due to type 1 multiple endocrine neoplasia. A newly recognized association.
    Am J Surg Pathol. 14 (6): 503-13.
  6. Gil-Loyzaga, P. et al. (1997) Serotonergic innervation of the organ of Corti of the cat cochlea.
    Neuroreport. 8: 3519-22.
  7. La Rosa, S. et al. (2004) Localization of inhibins and activins in normal endocrine cells and endocrine tumors of the gut and pancreas: an immunohistochemical and in situ hybridization study.
    J Histochem Cytochem. 52 (2): 217-25.
  8. La Rosa, S. et al. (2011) Histologic characterization and improved prognostic evaluation of 209 gastric neuroendocrine neoplasms.
    Hum Pathol. 42: 1373-84.
  9. View The Latest Product References
  10. Dainese, E. et al. (2013) Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.
    Pathol Res Pract. 209: 670-3.
  11. Vanoli, A. et al. (2013) Histologic changes in type A chronic atrophic gastritis indicating increased risk of neuroendocrine tumor development: the predictive role of dysplastic and severely hyperplastic enterochromaffin-like cell lesions.
    Hum Pathol. 44: 1827-37.
  12. Takeda, A. et al. (2015) Axonal regeneration through the fibrous scar in lesioned goldfish spinal cord.
    Neuroscience. 284: 134-52.
  13. Ismail, H.I. & Ali, A.M. (2015) Immunohistochemical Studies on The Endocrine Cells in The Thymus of The One-Humped-Camel (Camelus dromedarius)
    J Camel Pract Res. 22 (2): 251.

Synonyms
5-HT
5-HYDROXYTRYPTAMINE
RRID
AB_2122542

8250-0495

148504 152753 155922 157264 159947 164285 165274

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