p38 MAPK (pThr180/pTyr182) Antibody

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p38 MAPK (pThr180/pTyr182) Antibody gallery image 1

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Rabbit anti p38 MAPK (pThr180/Tyr182) used for western blotting of newt (Notophthalmus viridescens) mesemchymal derived A1 cell extracts.
Image captionActivation of JNK, p38 and c-Fos during myotube S-phase reentry (A, C) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or at different times post induction with 10%FCS. ERK indicates treatment with an ERK inhibitor, JNK/p38 denote treatment with either a JNK or p38 inhibitor respectively. Treatments were initiated at 0h post induction. (B) Western blot analysis of A1 myotube extracts 1 hour post serum induction, treated with the indicated inhibitors. Note that the ERK inhibitor specifically abrogates ERK phosphorylation. Inhibition of BMK1/ERK5 promotes S phase re-entry by decreasing A1 mononucleate proliferation (D) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or 1 hour post serum induction. (E)Representative image of A1 myotubes after 2d in high serum stained with antibodies against p-RBS807/811, MyHC and Hoechst 33258. (F) Representative image of A1 myotubes at 3d post-induction in high serum following a BrdU pulse. Myotubes were stained with antibodies against BrdU and Hoechst 33258. (G,H) Quantification of BrdU positive mononucleate cells (G) or combined cultures (H), as measured by immunostaining at 72h post serum induction following a BrdU pulse. Cells were treated with the indicated compounds. In (H), myotubes were induced and 30% confluent A1 proliferating cells were added where indicated (A1). All values represent the mean ± s.e.m (*p<0.05). n=3 (A-D), n=4 (G,H), were n indicates the number of independent experiments.

From: Yun MH, Gates PB, Brockes JP. Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts. Stem Cell Reports. 2014 Jun 19;3(1):15-23.

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p38 MAPK (pThr180/pTyr182) Antibody gallery image 2

Western blot of HeLa cell lysate that had been treated with +/- UV (30 min) showing phosphospecific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180 and Tyr182

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  • Rabbit anti p38 MAPK (pThr180/pTyr182)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP905WB*datasheet pdfdatasheet pdf0.1 ml
    AHP905
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Rat p38 MAPK (pThr180/pTyr182) antibody recognizes mitogen-activated protein kinase p38 (p38 MAPK), also known as mitogen-activated protein kinase 14 (MAPK 14), when phosphorylated at threonine 180 and tyrosine 182.

      p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.

      p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ChickenExpected from Sequence
      ZebrafishExpected from Sequence
      Humanyes
      BovineExpected from Sequence
      MonkeyExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      Newtyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated rat p38 MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated threonine180 and tyrosine 182.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      response to glucose stimulus
      protein C-terminus binding
      soluble fraction
      MAP kinase activity
      nucleus
      ATP binding
      stress-activated MAPK cascade
      protein autophosphorylation
      cytosolic part
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP905 recognises a band of approximately 39kD in Western blots of anisomycin C-6 glioma cell lysates.
    • Instructions For Use

    Additional p38 MAPK (pThr180/pTyr182) Antibody Formats

    Formats Applications Sizes available
    p38 MAPK (pThr180/pTyr182) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

              Further Reading

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