IL-10
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Recombinant Bovine Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.
This product is a purified recombinant cytokine corresponding to a 160 amino acid sequence of bovine IL-10 and is suitable for use as standard in the development of ELISA assays.
This product is a purified recombinant cytokine corresponding to a 160 amino acid sequence of bovine IL-10 and is suitable for use as standard in the development of ELISA assays.
Product Details
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 100μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE
- Protein Molecular Weight
- 18.6 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43480
- Entrez Gene
- IL10
- GO Terms
- GO:0002237 response to molecule of bacterial origin
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0002904 positive regulation of B cell apoptosis
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0030889 negative regulation of B cell proliferation
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032800 receptor biosynthetic process
- GO:0042095 interferon-gamma biosynthetic process
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045019 negative regulation of nitric oxide biosynthetic process
- GO:0045077 negative regulation of interferon-gamma biosynthetic process
- GO:0050715 positive regulation of cytokine secretion
- GO:0051045 negative regulation of membrane protein ectodomain proteolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- Regulatory
- For research purposes only
Applications of IL-10
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine Interleukin-10 | MCA2110 | E ES F* | 0.5 mg |
|
|
| Mouse anti Bovine Interleukin-10:Biotin | MCA2111B | E | 0.25 mg |
|
|
| Mouse anti Bovine Interleukin-10:Low Endotoxin | MCA2111EL | E F* FN | 0.5 mg |
|
|
| Mouse anti Bovine Interferon Gamma | MCA2112 | E ES F* | 0.5 mg |
|
Product Specific References
References for IL-10
-
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268.
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