IL-10

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Recombinant Bovine Interleukin-10

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PBP016A Datasheet	E SDS	25 µg	Log in		Get Quote

Recombinant Bovine Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

This product is a purified recombinant cytokine corresponding to a 160 amino acid sequence of bovine IL-10 and is suitable for use as standard in the development of ELISA assays.

Product Details

Target Species: Bovine
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 100μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE
Protein Molecular Weight: 18.6 kDa

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee: 12 months from date of despatch

More Information

UniProt: P43480
Entrez Gene: IL10
GO Terms: GO:0002237 response to molecule of bacterial origin; GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0002904 positive regulation of B cell apoptosis; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0030889 negative regulation of B cell proliferation; GO:0032715 negative regulation of interleukin-6 production; GO:0032800 receptor biosynthetic process; GO:0042095 interferon-gamma biosynthetic process; GO:0043193 positive regulation of gene-specific transcription; GO:0045019 negative regulation of nitric oxide biosynthetic process; GO:0045077 negative regulation of interferon-gamma biosynthetic process; GO:0050715 positive regulation of cytokine secretion; GO:0051045 negative regulation of membrane protein ectodomain proteolysis; GO:0051091 positive regulation of transcription factor activity; GO:0051384 response to glucocorticoid stimulus
Regulatory: For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Mouse anti Bovine Interleukin-10	MCA2110	E ES F*	0.5 mg	Log in	Get Quote
Mouse anti Bovine Interleukin-10:Biotin	MCA2111B	E	0.25 mg	Log in	Get Quote
Mouse anti Bovine Interleukin-10:Low Endotoxin	MCA2111EL	E F* FN	0.5 mg	Log in	Get Quote
Mouse anti Bovine Interferon Gamma	MCA2112	E ES F*	0.5 mg	Log in	Get Quote

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