IL-10

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Recombinant Bovine Interleukin-10

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP016A E 25 µg
Recombinant Bovine Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

This product is a purified recombinant cytokine corresponding to a 160 amino acid sequence of bovine IL-10 and is suitable for use as standard in the development of ELISA assays.

Product Details

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE
Protein Molecular Weight
18.6 kDa

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch

More Information

UniProt
P43480 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine Interleukin-10 MCA2110 0.5 mg E ES F*
Mouse anti Bovine Interleukin-10:Biotin MCA2111B 0.25 mg E
Mouse anti Bovine Interleukin-10:Low Endotoxin MCA2111EL 0.5 mg E F* FN
Mouse anti Bovine Interferon Gamma MCA2112 0.5 mg E ES F*